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  • 1
    ISSN: 1432-0789
    Keywords: Phospholipids ; Microbial biomass ; Phosphatase ; Cropping systems ; Long-term experiments Prairie
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In agricultural ecosystems that have had consistent cropping histories, standard microbial methods may be used to evaluate past and present practices. Our objective was to evaluate several microbial methods that best indicate cropping histories and soil quality on long-term plots. We selected soil microbial carbon (C), phospholipid analyses, direct counts of total fungal and bacterial biomass, and soil enzymes (phosphatases) to measure direct and indirect microbial activity on the Sanborn Field and Tucker Prairie. The Sanborn Field has been under various cropping and management practices since 1888 and the Tucker Prairie is an uncultivated site. Seven different plots were chosen on the Sanborn Field and random samples were taken in the summit area on the Tucker Prairie, which represented a reference site. Soil microbial biomass C, phospholipids, and enzyme activity were reflective of the cropping and management histories observed on the Sanborn Field. Enzymatic activity was highly correlated to soil organic matter. The direct counts of fungal and bacterial biomass showed that fungal populations dominated these soils, which may be attributed to soil pH. Soil microbial biomass C and enzyme assays seemed to be better potential indicators of cropping histories than the other methods tested in the long-term plots.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-0789
    Keywords: 15N transformations ; Crop residues ; Soil texture ; Soil aggregation ; Microbial pool ; Microbial biomass
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In a greenhouse pot study, we examined the availability of N to grain sorghum from organic and inorganic N sources. The treatments were15N-labeled clover residues, wheat residues, and fertilizer placed on a sandy clay loam and loamy sand soil surface for an 8-week period. Soil aggregates formed under each soil texture were measured after 8 weeks for each treatment. Significantly greater 15N was taken up and recovered by grain sorghum in sandy clay loam pots compared with loamy sand pots. Greater 15N recovery was consistently observed with the inorganic source than the organic sources regardless of soil texture or time. Microbial biomass C and N were significantly greater for sandy clay loam soil compared with the loamy sand. Microbial biomass 15N was also significantly greater in the sandy clay loam treatment compared to the loamy sand. The fertilizer treatment initially had the greatest pool of microbial biomass 15N but decreased with time. The crop residue treatments generally had less microbial biomass 15N with time. The crop residues and soil texture had a significant effect on the water-stable aggregates formed after 8 weeks of treatments. Significantly greater water-stable aggregates were formed in the sandy clay loam than the loamy sand. Approximately 20% greater water-stable aggregates were formed under the crop residue treatments compared to the fertilizer only treatment. Soil texture seemed to be one of the most important factors affecting the availability of N from organic or inorganic N sources in these soils.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-0789
    Keywords: Key words15N transformations ; Crop residues ; Soil texture ; Soil aggregation ; Microbial pool ; Microbial biomass
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In a greenhouse pot study, we examined the availability of N to grain sorghum from organic and inorganic N sources. The treatments were 15N-labeled clover residues, wheat residues, and fertilizer placed on a sandy clay loam and loamy sand soil surface for an 8-week period. Soil aggregates formed under each soil texture were measured after 8 weeks for each treatment. Significantly greater 15N was taken up and recovered by grain sorghum in sandy clay loam pots compared with loamy sand pots. Greater 15N recovery was consistently observed with the inorganic source than the organic sources regardless of soil texture or time. Microbial biomass C and N were significantly greater for sandy clay loam soil compared with the loamy sand. Microbial biomass 15N was also significantly greater in the sandy clay loam treatment compared to the loamy sand. The fertilizer treatment initially had the greatest pool of microbial biomass 15N but decreased with time. The crop residue treatments generally had less microbial biomass 15N with time. The crop residues and soil texture had a significant effect on the water-stable aggregates formed after 8 weeks of treatments. Significantly greater water-stable aggregates were formed in the sandy clay loam than the loamy sand. Approximately 20% greater water-stable aggregates were formed under the crop residue treatments compared to the fertilizer only treatment. Soil texture seemed to be one of the most important factors affecting the availability of N from organic or inorganic N sources in these soils.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1573-0646
    Keywords: gemcitabine ; 5-fluorouracil ; leucovorin ; phase I study
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Medicine
    Notes: Abstract Introduction: This was a dose escalation phase I trial designed to establish the MTD (maximum tolerated dose) and toxicity profile of the combination of gemcitabine, leucovorin and 5-fluorouracil (5-FU). Methods: Standard eligibility criteria were required for patients with advanced malignancy to enrol. Gemcitabine was escalated from an initial dose of 800 mg/m2. Gemcitabine was administered prior to leucovorin (25 mg/m2) followed by bolus 5-FU (600 mg/m2) every week for 3 weeks followed by 1 week of rest. Results: Of 21 patients enrolled, 20 were eligible for MTD determination. Patients received a median of three 4-week cycles of chemotherapy (range: 1 to 8 cycles). Toxicity was predominantly hematologic or gastroenterologic. Four dose levels were studied. At a gemcitabine dose of 1,500 mg/m2 systemic symptoms of fatigue accompanied hematologic toxicity and patients refused further therapy. At 1,250 mg/m2, full dose intensity was not delivered during the first cycle in 7 of 8 patients treated. Therefore, 1,000 mg/m2 was established as the recommended phase II dose for gemcitabine in this study. Antitumor activity was seen at all dose levels. Conclusions: The combination of gemcitabine, leucovorin and 5-FU was tolerable at full doses of all 3 drugs with an expected toxicity profile. Recommended phase II dose for gemcitabine was 1,000 mg/m2. Initial evidence of clinical activity was seen in a variety of tumor types.
    Type of Medium: Electronic Resource
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