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  • 6-Mercaptopurine  (1)
  • Accreditation of forensic laboratories  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Accreditation and quality assurance 1 (1996), S. 201-206 
    ISSN: 1432-0517
    Keywords: Key words Forensic science ; Toxicological analysis ; Quality assurance ; Round robins ; Accreditation of forensic laboratories
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract  In this paper, we review the work done in forensic toxicological analysis during about the last 30 years in Europe and to some extent in other parts of the world to assure the quality of toxicological investigations. Guidelines for carrying out toxicological analyses set up by professional bodies in the United States (AAFS/SOFT), Europe (GTFCh) and worldwide (TIAFT) are mentioned, and the problems of complying with the regulations set up in EN 45 000/ISO 9000 are discussed in detail. There are still diverging opinions as to whether toxicological laboratories should comply with good laboratory practice or with EN 45 000, and, as fas as Germany, Switzerland and Austria are concerned, a working group of the GTFCh is discussing which procedure should be adopted. Apart from having this discussion, the group aims to standardize suitable methods and is planning the organization of suitable round robins.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Research in experimental medicine 181 (1982), S. 19-25 
    ISSN: 1433-8580
    Keywords: Gas chromatography ; Serum level ; Azathioprine ; 6-Mercaptopurine ; Immunosuppression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The knowledge of serum levels of azathioprine or its metabolite 6-mercaptopurine is important for the monitoring of the immunosuppressive therapy after kidney transplantation, especially when kidney function is low. For this reason, the work of Bailey et al. was modified. 6-Mercaptopurine was extracted from the serum with a mixture of isopropanol-ethylacetate (1:1 v:v), derivatized with trimethylaniliniumhydroxyde and measured gaschromatographically. Sulfhydryl-protecting reagents were added to prevent loss of substance. Oxyethyltheophylline was used as the internal standard. The extraction and clean-up method recovered 80% of the 6-mercaptopurine present, the lower limit of sensitivity was 20 ng/ml, i.e., ten times lower than that of Bailey et al. The method could be realized on eight patients treated with azathioprine, the half life of the drug was about 20–40 min within the first hour after i.v. application.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
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