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  • 1
    Electronic Resource
    Electronic Resource
    Nucleotide sequence of the Aspergillus niger trpC gene: structural relationship with analogous genes of other organisms (1988)
    Springer
    Current genetics 13 (1988), S. 137-144 
    Details
    ISSN: 1432-0983
    Keywords: A. niger trpC gene ; Sequence analysis ; Amino acid homology ; Conservation ; Corrected A. nidulans trpC DNA sequence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The nucleotide sequence of the Aspergillus niger tryptophan C (trpC) gene was determined. Northern hybridization and S1-mapping experiments showed the presence of a 2.6 kb trpC poly(A)+ RNA with two very short (5 and 6 nucleotides) noncoding 5′-regions. Comparison of the predicted amino acid sequence with that of trp gene proteins of pro- and eukaryotic organisms revealed three functional domains (G, C, F) in the A. niger TrpC protein which catalyse the glutarnine amidotransferase reaction (GAT), the indole-3-glycerol phosphate synthase reaction (IGPS) and the N-(5′-phosphoribosyl) anthranilate isomerase reaction (PRAI), respectively. These domains are highly conserved and bordered by short areas showing less homology. Within the F domain of the trpC gene in A. niger, A. nidulans and Neurospora crassa, a region encoding 30 amino acids was found which is absent in the analogous genes of Saccharomyces cerevisiae and prokaryotic organisms. This region has features of a mutated in-phase intron.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00365648
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  • 2
    Electronic Resource
    Electronic Resource
    Development of a homologous transformation system for Aspergillus niger based on the pyrG gene (1987)
    Springer
    Molecular genetics and genomics 206 (1987), S. 71-75 
    Details
    ISSN: 1617-4623
    Keywords: A. niger homologous transformation ; A. niger pyrG gene ; A. nidulans gpd-lacZ fusion gene ; Cotransformation ; Heterologous expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The development of a homologous transformation system for Aspergillus niger is described. The system is based on the use of an orotidine-5′-phosphate decarboxylase deficient mutant (pyrG) and a vector, pAB4-1, which contains the functional A. niger pyrG gene as a selection marker. Transformation of the A. niger pyrG mutant with pAB4-1 resulted in the appearance of stable Pyr+ transformants at a frequency of 40 transformants per μg of DNA. In 90% of these transformants integration had occurred at the resident pyrG locus, resulting either in replacement of the mutant allele by the wild-type allele (60%) or in insertion of one or two copies of the vector (40%). The A. niger pyrG mutant could also be transformed with the vector pDJB2 containing the pyr4 gene of Neurospora crassa, at a frequency of 2 transformants per μg of DNA. Integration at the resident pyrG locus was not found with this vector. The vector pAB4-1 is also capable of transforming an Aspergillus nidulans pyrG mutant to Pyr+. The pyrG transformation system was used for the introduction of a non-selectable gene into A. niger.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00326538
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Transformation of Aspergillus oryzae using the A. niger pyrG gene (1987)
    Springer
    Molecular genetics and genomics 210 (1987), S. 460-461 
    Details
    ISSN: 1617-4623
    Keywords: Aspergillus oryzae ; Transformation ; A. niger pyrG gene ; Cotransformation ; Phleomycin ; Escherichia coli lacZ gene
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A transformation system for Aspergillus oryzae based on the orotidine-5′-phosphate decarboxylase gene (pyrG) was developed. Transformation frequencies of up to 16 transformants per μg of DNA were obtained with the vector pAB4-1, which carries the pyrG gene of A. niger. Southern blotting analysis showed that vector DNA sequences were integrated into the chromosomal DNA, in various copy numbers and presumably at different sites. Efficient cotransformation of an unselectable gene was also shown. Under the conditions used no transformants were obtained with the equivalent pyr4 gene of Neurospora crassa.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00327197
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    Paper (German National Licenses)
    Fulltext
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