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  • ATPase cytochemistry  (2)
  • Suaeda  (2)
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  • 1
    Digitale Medien
    Digitale Medien
    Quantitative ion localization within Suaeda maritima leaf mesophyll cells (1981)
    Springer
    Planta 151 (1981), S. 555-560 
    Details
    ISSN: 1432-2048
    Schlagwort(e): Halophyte ; Ion accumulation ; Suaeda
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Grown under saline conditions, Suaeda maritima accumulates Na+ and Cl- into its leaves, where individual mesophyll cells behave differently in their compartmentation of these ions. Measurements of ion concentrations within selected subcellular compartments show that freeze-substitution with dry sectioning is a valuable preparative technique for analytical electron microscopy of highly vacuolate plant material. Using this approach, absolute estimates were made of Na+, K+ and Cl- concentrations in the cytoplasm, cell walls, chloroplasts and vacuoles of leaf mesophyll cells.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00387435
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Evidence for the cytoplasmic localization of betaine in leaf cells of Suaeda maritima (1978)
    Springer
    Planta 140 (1978), S. 59-62 
    Details
    ISSN: 1432-2048
    Schlagwort(e): Betaine ; Lophytes ; Salt tolerance ; Suaeda
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract An attempt has been made to localize glycinebetaine in shoots of Suaeda maritima L. Dum. using a technique based on the formation of an iodoplatinate precipitate. Deposits were largely restricted to the cytoplasm of salt-grown plants and were analysed by transmission analytical electron microscopy. The results are considered to support the hypothesis that glycinebetaine acts as a cytoplasmic osmoticum to balance high vacuolar salt levels in certain halophytes.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00389380
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  • 3
    Digitale Medien
    Digitale Medien
    Cytochemical localization of ATPase activity in phloem tissues ofRicinus communis (1980)
    Springer
    Protoplasma 104 (1980), S. 55-65 
    Details
    ISSN: 1615-6102
    Schlagwort(e): ATPase cytochemistry ; Phloem ; Ricinus communis
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Standard lead precipitation procedures have been used to examine the localization of ATPase activity in phloem tissues ofRicinus communis. Reaction product was localized on the plasma membrane of the companion cells associated with sieve elements and of parenchyma cells in phloem tissues from the leaf, petiole, stem and root. ATPase activity was also present on the plasma membrane and dispersed P-protein of sieve elements in petiole, stem and root tissue, but was absent from the plasma membrane of these cells in the leaf minor veins. Substitution ofβ-glycerophosphate for ATP produced no change in the localization of reaction product in leaf tissue. These findings are discussed in relation to current theories on the mechanism of sugar transport and phloem loading.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01279368
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  • 4
    Digitale Medien
    Digitale Medien
    The validity of the lead precipitation technique for the localization of ATPase activity in plant cells (1980)
    Springer
    Protoplasma 104 (1980), S. 193-200 
    Details
    ISSN: 1615-6102
    Schlagwort(e): Analytical electron microscopy ; ATPase cytochemistry ; Plasma membrane ; Ricinus communis ; Triticum aestivum
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The claim that osmium-containing deposits which lack lead are frequently and incorrectly interpreted as enzymatic reaction products in lead precipitation techniques for ATPase localization in plants is without foundation. Proper controls clearly demonstrate the enzymatic origin of membrane-located deposits and the presence of lead is confirmed by analytical electron microscopy.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01279767
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