ISSN:
1432-203X
Keywords:
Abbreviations: 4PU, N-(2-chloro-4-pyridyl)-N'-phenylurea; BA, 6-benzyladenine; NAA, naphthalene acetic acid; B5, Gamborg et al. (1968)'s basal medium; PVP, polyvinylpyrrolidone.
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract: Nodule cultures were induced from shoot tips of aseptically grown Eucalyptus botryoides in a vertically rotated incubator. N-(2-chloro-4-pyridyl)-N'-phenylurea (4PU) was a very effective cytokinin for induction of nodules in liqiud B5 basal medium supplemented with naphthalene acetic acid and 3% sucrose. The effective concentration of 4PU ranged from 0.2 to 0.5mg/l. The nodule had a very smooth surface and was composed of small meristematic cells outside and large vacoulated cells inside. Shoots were regenerated from these cultures on media supplemented with 6-benzyladenine at 0.2mg/l in place of 4PU. These regenerated shoots were successfully rooted and cultivated in the field. Nodule cultures were maintained for 3–4 years with monthly subcultures without losing proliferation and regeneration abilities. Nodules were also successfully induced from other eucalypts, namely E. camaldulensis, E. deglupta and E. grandis with slightly modified media. Furthermore, nodule cultures were also induced from shoot tips of field-grown plants in E. camaldulensis. This system is benefical for both mass propagation of selected elite clones and creation of genetically engineered plants.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002990050172
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