ISSN:
1432-072X
Keywords:
Denitrifier
;
EPR
;
Nitric oxide
;
Cytochrome c′
;
Nitrosylheme
;
Achromobacter xylosoxidans
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The denitrifying bacterium, Achromobacter xylosoxidans NCIB 11015, was cultivated in meat extract-peptone medium and in Mn-free synthetic medium under denitrifying or non-denitrifying conditions. Electron paramagnetic resonance (EPR) spectra for the whole cells of the bacteria thus obtained were measured at 77K. The characteristic three-line signal was observed in the whole cells of the bacteria under denitrifying conditions, but not under non-denitrifying conditions. The three-line signal was more distinctly observed in the cells cultured in Mn-free medium. This signal could be assigned to nitrosylcytochrome c′ containing a five-coordinated nitrosylheme. The elemental composition in these cells is also discussed.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00245312
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