ISSN:
1432-1912
Keywords:
Neuroblastoma cells N2A
;
Acridine orange-staining
;
Cell differentiation
;
RNA distribution
;
Tissue culture
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary Vital staining of neuroblastoma cells with acridine orange produces a bright intracellular red-orange fluorescence most probably due to the occurrence of RNA. The distribution of this fluorescence depends on the state of morphological differentiation. The fluorescence is predominantly found in the perikaryon, the growth cones, and the endings of the processes of differentiated cells. This is of special interest in respect to the biochemistry of differentiation and the function of nerve cells. Comparative autoradiographical studies with 3H-uridine demonstrate that the newly synthesised RNA is transported into the endings of the cell processes.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00500897
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