ISSN:
1432-203X
Keywords:
Adventitious bud formation
;
Horseradish (Armoracia rusticana)
;
Leaf
;
Light
;
Phytohormone
;
Root
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary To clarify that the presence of Ri T-DNA genes are not prerequisite for the light-induced bud formation in horseradish (Armoracia rusticana) hairy roots, leaf and root segments of nontransformed horseradish plants were used as explants. Bud formation from nontransformed tissues was observed in hormone-free medium under 16 h daylight conditions, but not under continuous darkness. To investigate the effects of growth regulators on bud formation, leaf and root explants were treated with auxin (1-naphthaleneacetic acid; NAA) and / or cytokinin (6-benzyl-aminopurine; BA). The most effective treatment in the dark to stimulate bud formation was BA at 1 mg·1-1. These results show that adventitious bud formation in horseradish can be induced by light and growth regulators, and especially cytokinin, may be involved in bud formation, irrespective of whether the tissues were transformed with Ri T-DNA.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00232723
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