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  • 1
    Electronic Resource
    Electronic Resource
    Plasmid RNA polymerase-like mitochondrial sequences inAgaricus bitorquis (1996)
    Springer
    Current genetics 29 (1996), S. 370-376 
    Details
    ISSN: 1432-0983
    Keywords: Plasmid ; RNA polymerase ; Mitochondrion ; Agaricus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A linear mitochondrial plasmid, pEM, found in certain isolates of the basidiomyceteAgaricus bitorquis potentially encodes virus-like DNA and RNA polymerases. Mitochondrial DNA fromAgaricus bisporus that hybridizes to an internal region of pEM contains a fragmented and potentially non-functional version of the carboxy terminal end of the plasmid RNA polymerase. In this study, we present the sequence of the corresponding region of mitochondrial DNA fromA. bitorquis. This sequence contained the same region of the plasmid RNA polymerase gene as was reported for the mitochondrial DNA ofA. bisporus, and the level of similarity between theA. bisporus andA. bitorquis mitochondrial sequences was much higher than the level of similarity between either mitochondrial sequence and the plasmid. We propose that this plasmid RNA polymerase-like sequence was present in theAgaricus mitochondrial genome before the divergence ofA. bisporus andA. bitorquis, and thus is unlikely to be a recent derivative of the plasmid pEM.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02208618
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Plasmid-like DNAs in the commercially important mushroom genus Agaricus (1984)
    Springer
    Current genetics 8 (1984), S. 615-619 
    Details
    ISSN: 1432-0983
    Keywords: Agaricus ; Plasmid-like DNAs ; Mitochondrial DNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Two unique plasmid-like DNA components were localized in isolated mitochondria of the commercially important mushroom genus Agaricus: pEM (7.35 ± 0.15 kilobases) and pMPJ (3.65 ± 0.15 kilobases). These DNA moieties were linear; pEM possessed regions of terminal inverted repeated sequences. No homology was detected between pEM or pMPJ DNA and the nuclear or mitochondrial genomes. No homology existed between pEM and pMPJ. This suggests independent replication of pEM and pMPJ. Restriction endonuclease digests indicated that pEM consisted of two components (pEM1 and pEM2) with uniquely different restriction sites and copy number.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00395707
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    The mitochondrial DNAs of Agaricus: heterogeneity in A. bitorquis and homogeneity in A. brunnescens (1985)
    Springer
    Current genetics 9 (1985), S. 127-132 
    Details
    ISSN: 1432-0983
    Keywords: Agaricus ; Mitochondrial DNA ; Restriction pattern polymorphism ; Restriction endonuclease analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Mitochondrial DNAs were isolated from four cultivated strains of the commercial two-spored mushroom Agaricus brunnescens (bisporus) and from ten isolates of the four spored mushroom Agaricus bitorquis. Digestion of the fungal mitochondrial DNA with restriction endonucleases yielded numerous fragments. Summation of the fragment sizes gave a mitochondrial DNA size of 98.3 ± 2.4 kilobases (kb) (64.9 x 106 daltons) for A. brunnescens. The size of the mitochondrial DNA ranged from 148.5 ± 10.8 kb (98.0 x 106 daltons) to 176.3 ± 12.0 kb (116.4 x 106 daltons) for A. bitorquis. The restriction patterns, produced by a variety of endonucleases, were identical for all four isolates of A. brunnescens. The ten isolates of A. bitorquis demonstrated extensive restriction pattern heterogeneity and have been tentatively assigned into four groups. Approximately 60% of the A. bitorquis mitochondrial DNA restriction fragments show sequence homology with A. brunnescens mitochondrial DNA based on DNA — DNA hybridizations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00436960
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Homology between mitochondrial DNA of Agaricus bisporus and an internal portion of a linear mitochondrial plasmid of Agaricus bitorquis (1991)
    Springer
    Current genetics 19 (1991), S. 495-502 
    Details
    ISSN: 1432-0983
    Keywords: Agaricus ; Mitochondria ; Plasmid ; RNA polymerase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Agaricus bisporus, the cultivated mushroom, contains a mitochondrial fragment (50H) which was previously demonstrated by Southern hybridization to have sequence similarity to an internal region of pEM, a linear mitochondrial plasmid of Agaricus bitorquis. The nucleotide sequence of 50H was determined and compared to the sequence of the corresponding pEM fragment. The region of sequence homology on pEM is contained within an open reading frame (ORF) that may encode an RNA polymerase, but 50H is neither an intact nor a complete copy of the ORF. pEM also contains an ORF with characteristics of genes for virus-encoded DNA polymerases. pEM appears to be very similar to other linear mitochondrial plasmids (in fungi and higher plants) reported to contain ORFs that may encode the same types of polymerases. The potential functionality of the pEM sequence suggests that it has diverged less than the mitochondrial fragment from a common ancestor.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00312742
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  • 5
    Electronic Resource
    Electronic Resource
    Physical mapping of the mitochondrial genome of the cultivated mushroom Agaricus brunnescens (= A. bisporus) (1988)
    Springer
    Current genetics 14 (1988), S. 43-49 
    Details
    ISSN: 1432-0983
    Keywords: Agaricus ; MtDNA ; Restriction map ; Inverted repeat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Mitochondrial (mt) DNA from the commercial mushroom Agaricus brunnescens Peck [= A. bisporus (Lange) Imbach] was purified by cesium chloride/bisbenzimide gradient centrifugation. A physical map of the mtDNA fragments produced by BamHI, EcoRl, and PvuII digestion was generated by filter hybridizations with radiolabelled BamHI mtDNA probes. The A. brunnescens mtDNA was a circular molecule 136 kilo-basepairs (kbp) in length and contained an inverted repeat between 4.6 and 9.2 kbp in size. Orientational isomers of the mitochondrial genome were not detected. The positions of six genes were located on the A. brunnescens mtDNA map by heterologous hybridization. No coding function has yet been ascribed to the inverted repeat. The large rRNA gene was located on the smaller single copy region. The genes for cytochrome b, cytochrome oxidase (subunit III), ATPase (subunits 8 and 6) and the small rRNA were located on different regions of the larger single copy region.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00405852
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    Paper (German National Licenses)
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