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  • 1
    Electronic Resource
    Electronic Resource
    Agrobacterium and plant genetic engineering (1992)
    Springer
    Plant molecular biology 19 (1992), S. 15-38 
    Details
    ISSN: 1573-5028
    Keywords: Agrobacterium tumefaciens ; plant transformation ; plant tumours ; T-DNA ; Ti plasmid ; transgenic plants
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00015604
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Deviating T-DNA transfer fromAgrobacterium tumefaciens to plants (1996)
    Springer
    Plant molecular biology 31 (1996), S. 677-681 
    Details
    ISSN: 1573-5028
    Keywords: Agrobacterium tumefaciens ; Arabidopsis thaliana ; T-DNA transfer ; transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We analyzed 29 T-DNA inserts in transgenicArabidopsis thaliana plants for the junction of the right border sequences and the flanking plant DNA. DNA sequencing showed that in most lines the right border sequences transferred had been preserved during integration, corroborating literature data. Surprisingly, in four independent transgenic lines a complete right border repeat was present followed by binary vector sequences. Cloning of two of these T-DNA inserts by plasmid rescue showed that in these lines the transferred DNA consisted of the complete binary vector sequences in addition to the T-region. On the basis of the structure of the transferred DNA we propose that in these lines T-DNA transfer started at the left-border repeat, continued through the vector part, passed the right border repeat, and ended only after reaching again this left-border repeat.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00042239
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Studies on the structure of cointegrates between octopine and nopaline Ti-plasmids and their tumour-inducing properties (1982)
    Springer
    Plant molecular biology 1 (1982), S. 265-276 
    Details
    ISSN: 1573-5028
    Keywords: Southern hybridizations ; crown gall ; Nicotiana tabacum ; Agrobacterium tumefaciens ; Ti-plasmids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Stable cointegrates between incRh-1 octopine (Ach5) and nopaline (C58) Ti-plasmids, present in ten independently isolated Agrobacterium tumefaciens strains, showed identical restriction endonuclease patterns. Each cointegration event had taken place in the common sequence between the T-regions of both Ti-plasmids. This illustrates a high preference for this region when used in the formation of cointegrates. Four crown gall tissues, obtained after transformation of Nicotiana tabacum cells by one of the mutants, were analysed by using Southern blot analysis for their T-DNA structure. The borders of T-DNA frequently appeared to differ from T-DNA borders previously detected in tumour tissues that had been induced by Agrobacterium strain C58 or Ach5. Therefore, it was concluded that possibly a less stringent mechanism exists for the integration into plant DNA of T-DNA, derived from a composite (octopine/nopaline) T-region than for integration of T-DNA from a normal (octopine or nopaline) T-region.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00027558
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Octopine and nopaline strains of Agrobacterium tumefaciens differ in virulence; molecular characterization of the virF locus (1990)
    Springer
    Plant molecular biology 14 (1990), S. 249-259 
    Details
    ISSN: 1573-5028
    Keywords: Agrobacterium tumefaciens ; virulence gene ; host range ; plant tumour induction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Octopine and nopaline strains of Agrobacterium tumefaciens were found to differ in virulence on Nicotiana glauca. This difference is due to the absence of a functional virF locus, which is necessary for efficient tumorigenesis on N. glauca, from the nopaline Ti plasmids. Genetic studies and DNA sequence analysis of the virF locus revealed that virF embraces one open reading frame coding for a hydrophilic protein with a molecular mass of 22437 Da. Transcription of virF is directed from left to right, towards the T region, and is strongly induced by the phenolic compound acetosyringone. We established that virA and virG, two genes known to be essential for induction of the vir regulon, are necessary for acetosyringoneinduced virF expression, implying that virF is a member of this vir regulon. Agrobacterium virF mutants can be complemented for tumor induction by co-infection with avirulent Agrobacterium ‘helper’ strains. We found that such ‘helper’ strains must express not only the virF gene but also the vir operons virA, virB, virD and virG.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00018565
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    Paper (German National Licenses)
    Fulltext
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