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  • 1
    Electronic Resource
    Electronic Resource
    Effect of various barbituric acid derivatives on survival of functional hepatocytes from adult rats in primary culture (1987)
    Springer
    Research in experimental medicine 187 (1987), S. 105-117 
    Details
    ISSN: 1433-8580
    Keywords: Primary culture of adult rat hepatocytes ; Barbituric acid derivatives ; Hepatocyte survival ; Albumin secretion ; Tyrosine aminotransferase activity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Eighteen barbituric acid (BA) derivatives and three structurally related chemicals (non-BA-derivatives) were tested for their potency in supporting survival of functional hepatocytes from adult rats in primary culture. Of the 18 BA derivatives, nine drugs showed excellent maintenance effect on hepatocyte survival and function. Although four BA derivatives were also effective, their potency was relatively lower. The remaining five BA derivatives and three structurally related chemicals exhibited no maintenance effect. Thus, a correlation was found between the BA derivative structure and the potency for supporting hepatocyte survival in primary culture. The dose response curves of hepatocyte survival were generally biphasic in shape, as a function of BA derivative concentration. The optimum concentrations for observing the morphological and biochemical effects of the BA derivatives differed from each other. The maintenance of hepatocytes was attained only in the continuous presence of the BA derivatives in the medium. The nine excellent BA derivatives efficiently prevented hepatocytes from morphological degeneration which was observed in the control cultures. The surviving hepatocytes in the presence of these BA derivatives showed higher albumin secretion and retained higher basal levels of tyrosine aminotransferase (TAT) activity for at least 2 weeks in primary culture, as compared with control. Furthermore, the addition of dexamethasone (10 µM) caused a 2- to 4-fold induction of TAT activity for at least 2 weeks in primary culture.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01851972
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Lectin histochemistry of canine polyglucosan bodies (1987)
    Springer
    Acta neuropathologica 73 (1987), S. 177-180 
    Details
    ISSN: 1432-0533
    Keywords: Lectins ; Polyglucosan bodies ; Dog
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Lectin histochemistry was investigated to identify sugar residues of the polyglucosan bodies of canine brain, spinal cord and caecum. The polyglucosan bodies in the brain and spinal cord stained with concanavalin A (ConA) but not with soybean agglutinin, wheat germ agglutinin, peanut agglutinin,Dolichos biflorus agglutinin,Ricinus communis agglutinin andUlex europaeus agglutinin. Caecum, polyglucosan bodies, however, did not stain with any of the seven lectins employed. After periodate oxidation, paradoxical ConA staining was observed in the polyglucosan bodies of the brain, spinal cord and caecum. These results indicate that polyglucosan bodies contain mannose and glucose residues and suggest that the component of polyglucosan bodies is partially derived from rough endoplasmic reticulum, the Golgi apparatus and hypolemmal cisternae.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00693785
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    Paper (German National Licenses)
    Fulltext
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