ISSN:
1438-2199
Keywords:
Amino acids
;
Dansyl amino acids
;
Cyclodextrin
;
Capillary electrophoresis
;
Organic modifiers
;
Enantioselectivity
;
Chiral resolution
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary Using capillary electrophoresis, the enantiomers of a number of dansyl amino acids were resolved using nativeβ-cyclodextrin. The neutral chiral host resolved analytes possessing a negative charge at pH 9, the conditions employed in this study. Organic modifiers added to the running buffer were particularly adept at enhancing chiral recognition between the guest and host molecule in capillary electrophoresis. This work examined the effects of methanol, dimethylformamide, and acetonitrile on the resolution, migration time, and efficiency of twelve dansyl amino acids. Examples are given of the separation of racemic dansyl amino acids utilizing this technique and conditions necessary to achieve enantioselectivity.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00806551
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