ISSN:
1432-041X
Keywords:
Plasma membranes
;
Amphibian embryogenesis
;
Neural induction
;
Xenopus laevis
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Plasma membranes were isolated in high yield from Xenopus gastrulae by repeated sedimentation in discontinuous sucrose gradients. Most of the yolk was separated by lowspeed sedimentation before centrifugation on the discontinuous sucrose gradients. The isolation of plasma membranes was followed by covalent labelling of the surface of dissociated gastrula cells with diazoniobenzene sulphonate, by electron microscopy and the distribution of enzymatic markers. The isolated plasma membranes have a low neural inducing activity as compared to other cell constituents.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00456107
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