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  • Pancreatic islets  (2)
  • Analytical Chemistry and Spectroscopy  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Lymphokine release during co-culture of human lympho-mononuclear cells and fresh or cultured human, porcine and bovine pancreatic islets (1996)
    Springer
    Acta diabetologica 33 (1996), S. 122-125 
    Details
    ISSN: 1432-5233
    Keywords: Key words  Lymphokines ; Pancreatic islets
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract   In this study we evaluated whether isolated human (HI), porcine (PI) and bovine (BI) islets, either fresh (Fr) or cultured for 4 weeks (4w) affect cytokine release from human lymphomononuclear cells (LMC) differently. We prepared LMC from peripheral blood by density gradient purification and co-cultured 1×106 LMC for 24 h with 100 hand-picked islets, either within 48 h of isolation or after culture for 4 weeks. Soluble interleukin-2 receptor (IL-2R), interferon-gamma (IFN), interleukin-4 (IL-4) and interleukin-10 (IL-10) were measured by sandwich enzyme-linked immunoadsorbent assay. Compared with controls (Ctrl, LMC without islets), Fr-HI, Fr-PI and Fr-BI caused a similar increase of IL-2R and IFN release, whereas 4w-HI and 4w-BI did not lead to any significant production of these two cytokines. IL-10 concentrations increased with Fr-PI and Fr-BI, but not with Fr-HI, and no major effect of the 4-week culture was seen. IL-4 levels were below the detection limit of the method used in these experiments. Thus, fresh allo- and xeno-islets caused a similar increase of the release of cytokines known to be markers of Th1 activation, whereas the release of IL-10, a marker of Th2 activation, increased with xeno-, but not with allo-islets; culturing the islets for 4 weeks decreased Th1, but not Th2 activation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00569422
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Lymphokine release during co-culture of human lympho-mononuclear cells and fresh or cultured human, porcine and bovine pancreatic islets (1996)
    Springer
    Acta diabetologica 33 (1996), S. 122-125 
    Details
    ISSN: 1432-5233
    Keywords: Lymphokines ; Pancreatic islets
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract In this study we evaluated whether isolated human (HI), porcine (PI) and bovine (BI) islets, either fresh (Fr) or cultured for 4 weeks (4w) affect cytokine release from human lymphomononuclear cells (LMC) differently. We prepared LMC from peripheral blood by density gradient purification and co-cultured 1×106 LMC for 24 h with 100 hand-picked islets, either within 48 h of isolation or after culture for 4 weeks. Soluble interleukin-2 receptor (IL-2R), interferon-gamma (IFN), interleukin-4 (IL-4) and interleukin-10 (IL-10) were measured by sandwich enzyme-linked immunoadsorbent assay. Compared with controls (Ctrl, LMC without islets), Fr-HI, Fr-PI and Fr-BI caused a similar increase of IL-2R and IFN release, whereas 4w-HI and 4w-BI did not lead to any significant production of these two cytokines. IL-10 concentrations increased with Fr-PI and Fr-BI, but not with Fr-HI, and no major effect of the 4-week culture was seen. IL-4 levels were below the detection limit of the method used in these experiments. Thus, fresh allo- and xeno-islets caused a similar increase of the release of cytokines known to be markers of Th1 activation, whereas the release of IL-10, a marker of Th2 activation, increased with xeno-, but not with allo-islets; culturing the islets for 4 weeks decreased Th1, but not Th2 activation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00569422
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Raman and infrared spectroscopic study of the molecular characterization of the biocompatibility of prosthetic biomaterials (1994)
    Chichester [u.a.] : Wiley-Blackwell
    Journal of Raman Spectroscopy 25 (1994), S. 109-114 
    Details
    ISSN: 0377-0486
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: Raman spectra were obtained directly in a non-destructive and non-invasive way from the biomaterials PMMA [poly(methyl methacrylate)], HDPE (high-density polyethylene), α-Al2O3 (alumina) and HA (hydroxyapatite) constituting the components (bone cement, acetabular cup, ball and stem coated with ceramic) of a hip prosthesis. The aim was to give an objective contribution at a molecular level to predict their biocompatibility. In particular, Raman spectra of explanted PMMA bone cements do not show the 1640 cm-1 band due to the stretching mode of C=C unsaturated centres. Moreover, conformational changes of PMMA following the implant appear in the spectra in the range 1100-500 cm-1, where conformational marker bands at 985, 968, 601 and 565 cm-1 are present. The limited number of HDPE explanted acetabular cups did not allow unequivocal Raman results to be obtained by means of band deconvolution in the region between 1500 and 1000 cm-1, regarding the changes in the crystalline, amorphous and interfacial contents of the acetabular cup surfaces following the implant. α-Al2O3 proved to be a ceramic with good biocompatibility as a biomaterial constituting a hip prosthesis. As regards the stem coating, Raman and Fourier transform IR spectra of HA pre-coating and HA coating on metal implants show spectroscopic modifications due to structural and chemical changes with formation of other calcium phosphates. The development of new bioactive coatings containing precursors of bone growth and able to promote osteogenesis is outlined.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jrs.1250250116
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
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