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Temperature sensitive pet mutants in yeast Saccharomyces cerevisiae that lose mitochondrial RNA (1987)

Mueller, David M. ; Biswas, Tapan K. ; Backer, James ; [et al.]

Springer

Current genetics 11 (1987), S. 359-367

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ISSN: 1432-0983

Keywords: Yeast ; Mitochondrial ; Mutants ; RNA

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Summary This is a description of a new class of temperature sensitive pet mutants in Saccharomyces cereviase that lose all or part of their mitochondrial RNA at the restrictive temperature. These mutants fall into 8 different complementation groups, mna1 to mna8, and 2 different classes based on their phenotype. Class I mutations, mna1-1 through mna5-1, cause complete or partial loss of mitochondrial RNA at the restrictive temperature. The mutation, mna1-1, is especially interesting since it causes a loss of both mitochondrial DNA and RNA when the mutant is grown on a fermentable carbon source at the restrictive temperature. However, when this mutant is grown at the permissive temperature on a non-fermentable carbon source then shifted to the restrictive temperature, only the mitochondrial RNA is lost. This indicates that the primary cause for the pet phenotype is due to the loss of mitochondrial RNA and not DNA. Class II mutations, mna6-1 through man8-1, cause complete loss of the 14S rRNA after growth at the restrictive temperature in a fermentable carbon source. This loss appears to be specific for the 14S rRNA, since all other transcripts probed by Northern analysis are normal.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00378178

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Cross-polarization for quadrupolar nuclei - proton to molybdenum-95 (1990)

Edwards, John C. ; Ellis, Paul D.

Chichester : Wiley-Blackwell

Organic Magnetic Resonance 28 (1990), S. S59

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ISSN: 0749-1581

Keywords: 95Mo NMR ; Solid-state ; Cross-polarization ; Molybdates ; Chemistry ; Analytical Chemistry and Spectroscopy

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Chemistry and Pharmacology

Notes: High-field, 95Mo, solid-state nuclear magnetic resonance spectra of (NH4)6Mo7O24 · 4H2O and (Bu4N)2Mo2O7 were obtained using solid-echo techniques together with 1H cross-polarization and dipolar decoupling. The results show that reliable second-order powder line shapes can be obtained with excellent cross-polarization enhancements of 66-86% of maximum theoretical enhancement. The lower than maximum enhancements are due to the relatively long TIS values, which are similar in magnitude to the TH1ρ values. Experiments were performed which reveal the dynamics involved in the cross-polarization process, including measurement of the cross-relaxation time constant and the 1H and 95Mo rotating-frame spin-lattice relaxation times. It was found that the principal relaxation process causing low gain in the observed signal was the 1H T1ρ, which was found to be on the order of 23-24 ms. The experimental results obtained indicate that selective cross-polarization dynamics occur in molybdenum cluster species with inequivalent molybdenum sites. The 95Mo rotating-frame spin-lattice relaxation rate is long in comparison with the other relaxation processes, making molybdenum a good cross-polarization candidate but precluding the use of multiple contact cross-polarization.

Additional Material: 9 Ill.