ISSN:
0951-4198
Keywords:
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Physics
Notes:
A mock sequencing experiment is described in which matrix-assisted laser desorption/ionization (MALDI) mass spectrometry is used to analyze mixtures of synthetic oligodeoxynucleotides chosen to match the first 24 DNA fragments generated in typical sequencing reactions using a standard M13mp19 template and primer. The experiments permitted the evaluation of important issues relating to resolution, sensitivity, multiple charging, and adduct formation. Mass resolutions (full width at half maximum) were between 100 and 200, and it was relatively straightforward to assign bases in the sequencing simulation using subpicomolar quantities of material. These results demonstrate the potential of MALDI mass spectrometry in DNA sequence analysis.
Additional Material:
1 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/rcm.1290071008
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