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  • 1995-1999  (4)
  • Apolipoprotein B-100  (2)
  • low-density lipoprotein composition  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Oxidation of low-density lipoprotein in NIDDM: its relationship to fatty acid composition (1995)
    Springer
    Diabetologia 38 (1995), S. 1300-1306 
    Details
    ISSN: 1432-0428
    Keywords: Non-insulin-dependent diabetes mellitus ; low-density lipoprotein oxidation ; dietary fatty acids ; low-density lipoprotein composition ; glycated low-density lipoprotein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The increased risk of atherosclerotic disease in diabetic subjects may be due to enhanced foam cell formation following an increased susceptibility of low density lipoprotein to oxidative modification. This study has compared fatty acid content and lipoprotein oxidisability in 10 non-insulin-dependent diabetic subjects with that in 10 control subjects. Both groups were normocholesterolaemic and the diabetic subjects had higher triglyceride levels (2.2±0.4 vs 1.2±0.2 mmol/l, p〈0.05). The fatty acid composition was compared in low density lipoprotein following Folch extraction, separation by thin layer chromatography (for the lipid classes) and analysis by gas liquid chromatography. Low density lipoprotein oxidisability was assessed by conjugated diene and thiobarbituric acid reacting substance formation in the presence of copper ions. The esterified/free cholesterol ratio was higher in the low density lipoprotein from patients compared to control subjects (2.9±0.1 vs 1.9±0.3, p〈0.05). Linoleic acid in the cholesteryl ester fraction of the lipoprotein was higher in the patients than in the control subjects (48.2±2.2% vs 42.4±3.4%, p〈0.05) as was the total quantity of linoleic acid in the cholesteryl ester fraction (317.8±68.0 vs 213.2±28.0 Μg/mg protein, p〈0.05) and in the low-density lipoprotein as a whole (443.2±70.0 vs 340.2±28.2 Μg/mg protein, p〈0.05). Lipoprotein oxidisability was also increased in the diabetic group with increased formation of thiobarbituric acid reacting substances (35.6±7.2 vs 22.3±3.5 nmol/mg protein, p〈0.05, increased total diene formation (502±60 vs 400±30 nmol/mg protein, p〈0.05) and increased rate of diene formation (7.2±0.6 vs 5.1±0.9 nmol diene · mg protein−1 · min−1, p〈0.05). This study indicates that low-density lipoprotein from diabetic subjects is more susceptible to oxidation. This could, in vivo, accelerate foam-cell formation thereby increasing atherosclerotic risk in diabetic subjects.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00401762
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Oxidation of low-density lipoprotein in NIDDM: its relationship to fatty acid composition (1995)
    Springer
    Diabetologia 38 (1995), S. 1300-1306 
    Details
    ISSN: 1432-0428
    Keywords: Key words Non-insulin-dependent diabetes mellitus ; low-density lipoprotein oxidation ; dietary fatty acids ; low-density lipoprotein composition ; glycated low-density lipoprotein.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The increased risk of atherosclerotic disease in diabetic subjects may be due to enhanced foam cell formation following an increased susceptibility of low density lipoprotein to oxidative modification. This study has compared fatty acid content and lipoprotein oxidisability in 10 non-insulin-dependent diabetic subjects with that in 10 control subjects. Both groups were normocholesterolaemic and the diabetic subjects had higher triglyceride levels (2.2 ± 0.4 vs 1.2 ± 0.2 mmol/l, p 〈 0.05). The fatty acid composition was compared in low density lipoprotein following Folch extraction, separation by thin layer chromatography (for the lipid classes) and analysis by gas liquid chromatography. Low density lipoprotein oxidisability was assessed by conjugated diene and thiobarbituric acid reacting substance formation in the presence of copper ions. The esterified/free cholesterol ratio was higher in the low density lipoprotein from patients compared to control subjects (2.9 ± 0.1 vs 1.9 ± 0.3, p 〈 0.05). Linoleic acid in the cholesteryl ester fraction of the lipoprotein was higher in the patients than in the control subjects (48.2 ± 2.2 % vs 42.4 ± 3.4 %, p 〈 0.05) as was the total quantity of linoleic acid in the cholesteryl ester fraction (317.8 ± 68.0 vs 213.2 ± 28.0 μg/mg protein, p 〈 0.05) and in the low-density lipoprotein as a whole (443.2 ± 70.0 vs 340.2 ± 28.2 μg/mg protein, p 〈 0.05). Lipoprotein oxidisability was also increased in the diabetic group with increased formation of thiobarbituric acid reacting substances (35.6 ± 7.2 vs 22.3 ± 3.5 nmol/mg protein, p 〈 0.05, increased total diene formation (502 ± 60 vs 400 ± 30 nmol/mg protein, p 〈 0.05) and increased rate of diene formation (7.2 ± 0.6 vs 5.1 ± 0.9 nmol diene · mg protein–1· min–1, p 〈 0.05). This study indicates that low-density lipoprotein from diabetic subjects is more susceptible to oxidation. This could, in vivo, accelerate foam-cell formation thereby increasing atherosclerotic risk in diabetic subjects. [Diabetologia (1995) 38: 1300–1306]
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00401762
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Elevated triglyceride-rich lipoproteins in diabetes (1996)
    Springer
    Acta diabetologica 33 (1996), S. 205-210 
    Details
    ISSN: 1432-5233
    Keywords: Key words Apolipoprotein B-48 ; Apolipoprotein B-100 ; Triglyceride-rich lipoprotein ; Non-insulin-dependent ; diabetes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The role of the intestine in cholesterol metabolism in human diabetes is unclear, although abnormalities have been demonstrated in cholesterol synthesis and absorption in diabetic animals. This study examines the relationship between fasting and post-prandial apolipoprotein B-48 in type 2 (non-insulin-dependent) diabetic and non-diabetic subjects. Eight type 2 diabetic patients and ten healthy non-diabetic control subjects were given a high-fat meal (1300 kcal), and the triglyceride-rich lipoprotein fraction was isolated by ultracentrifugation (d〈1.006 g/ml) from fasting and post-prandial plasma. Apolipoprotein B-48 and apo B-100 were separated on 4%–15% gradient gels and quantified by densitometric scanning with reference to a purified low-density lipoprotein (LDL) apo B-100 preparation. Diabetic patients had significantly higher concentrations of apo B-48 and apo B-100 in both the fasting (P〈0.05) and post-prandial (P〈0.001) triglyceride-rich lipoprotein samples compared with non-diabetic subjects. The diabetic patients also exhibited a significantly different post-prandial profile for apo B-48 and apo B-100, with a prolonged increase and a later post-prandial peak, than the non-diabetic subjects (P〈0.01). These results suggest that the raised fasting triglyceride-rich lipoproteins, often found in diabetes, are associated with apo B-48 and may be derived from increased intestinal chylomicron production. The post-prandial pattern suggests an abnormality in intestinal production as well as hepatic clearance of apo B-48 in type 2 diabetes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02048544
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Elevated triglyceride-rich lipoproteins in diabetes (1996)
    Springer
    Acta diabetologica 33 (1996), S. 205-210 
    Details
    ISSN: 1432-5233
    Keywords: Apolipoprotein B-48 ; Apolipoprotein B-100 ; Triglyceride-rich lipoprotein ; Non-insulin-dependent diabetes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The role of the intestine in cholesterol metabolism in human diabetes in unclear, although abnormalities have been demonstrated in cholesterol synthesis and absorption in diabetic animals. This study examines the relationship between fasting and post-prandial apolipoprotein B-48 in type 2 (non-insulin-dependent) diabetic and non-diabetic subjects. Eight type 2 diabetic patients and ten healthy non-diabetic control subjects were given a high-fat meal (1300 kcal), and the triglyceride-rich lipoprotein fraction was isolated by ultracentrifugation (d〈1.006 g/ml) from fasting and post-prandial plasma. Apolipoprotein B-48 and apo B-100 were separated on 4%–15% gradient gels and quantified by densitometric scanning with reference to a purified low-density lipoprotein (LDL) apo B-100 preparation. Diabetic patients had significantly higher concentrations of apo B-48 and apo B-100 in both the fasting (P〈0.05) and post-prandial (P〈0.001) triglyceride-rich lipoprotein samples compared with non-diabetic subjects. The diabetic patients also exhibited a significantly different post-prandial profile for apo B-48 and apo B-100, with a prolonged increase and a later post-prandial peak, than the non-diabetic subjects (P〈0.01). These results suggest that the raised fasting triglyceride-rich lipoproteins, often found in diabetes, are associated with apo B-48 and may be derived from increased intestinal chylomicron production. The post-prandial pattern suggests an abnormality in intestinal production as well as hepatic clearance of apo B-48 in type 2 diabetes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02048544
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
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