ISSN:
1432-1912
Keywords:
Prostaglandin
;
Phospholipase A
;
Arachidonic Acid
;
Acetylcholine
;
Frog Intestine
;
Prostaglandin
;
Phospholipase A
;
Arachidonsäure
;
Acetylcholin
;
Froschdarm
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary 1. Frog intestines were perfused through their vascular system and the content of prostaglandin (PG) in the effluent was estimated biologically. 2. The spontaneous release of PG ranged from 0.55 to 29 ng PGE1 equivalents/min. Thin-layer chromatography indicated that the liberated compound was a PGE compound. 3. Acetylcholine and dimethyl-phenyl-piperazinium iodide raised the output of PG significantly. Adrenaline had no definite effect. 4. Lysolecithin weakly stimulated the release of PG, but phospholipase A caused an extraordinary rise which was apparently due to formation of new PGE1. 5. A considerable formation of PG was also seen when arachidonic acid was infused. Being PGE2 it obviously originated from the infused fatty acid. This fact demonstrates directly that a PG-forming enzyme system is active in the intact tissue. 6. It is suggested that physiological stimuli can increase the formation of PG by providing free precursor acids. This could be effected by the activation of an endogenous phospholipase A.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00997282
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