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  • 1
    Electronic Resource
    Electronic Resource
    Factors that influence Agrobacterium rhizogenes-mediated transformation of broccoli (Brassica oleracea L. var. italica) (2000)
    Springer
    Plant cell reports 19 (2000), S. 994-999 
    Details
    ISSN: 1432-203X
    Keywords: Key words Agrobacterium rhizogenes ; Brassica oleracea ; Transformation ; Arginine ; Acetosyringone
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  An improved broccoli transformation system was developed by optimising several factors that affect the rate of effective Agrobacterium-mediated transformation. Leaf explants of cultivar Shogun were co-cultivated with Agrobacterium rhizogenes strain A4T harbouring the binary vector pART278. The T-DNA of this binary vector contains a neomycin phosphotransferase II (NOS-NPTII-NOS) gene for kanamycin resistance and a β-glucuronidase (35S-GUS-OCS) gene. Several media and factors were evaluated including combinations of arginine, mannopine, acetosyringone and the use of feeder cell layers. The new protocol includes the use of 200 μm acetosyringone in LB medium for bacterial growth, the use of a Brassica campestris feeder cell layer, 10 mm mannopine and 50 μm acetosyringone in the co-cultivation medium and 1 mm arginine in the selection medium. The use of this optimised protocol produced transformation rates of 33% in preliminary experiments transforming broccoli with the antisense 1-aminocyclopropane-1-carboxylic acid (ACC) oxidase gene from pTOM13.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990000221
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  • 2
    Electronic Resource
    Electronic Resource
    IBPGR morphological descriptors — their relevance in determining patterns within a diverse spring barley germplasm collection (1992)
    Springer
    Theoretical and applied genetics 85 (1992), S. 489-495 
    Details
    ISSN: 1432-2242
    Keywords: IBPGR ; Morphological descriptors ; Genetic diversity ; Barley
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The International Board for Plant Genetic Resources (IBPGR) promotes a minimum set of morphological characters thought satisfactory for the custodial management of crop germplasm collections. The purpose of such conserved germplasm is as a genetic resource for future plant breeding programmes. Because future plant breeding requirements are not always known, the curator's strategy in maintaining an adequate germplasm resource is to conserve as wide a range of genetic diversity as possible. How is diversity measured to ensure a wide range of conserved germplasm? The IBPGR minimum descriptors detail genetic diversity at particular points in a genome corresponding to the observed characters. The purpose of the present study was to investigate whether diversity as identified by the IBPGR minimum set of descriptors could yield satisfactory measures of diversity in a contrasting set of genomic markers. A diverse spring-sown barley collection of 1379 cultivars was evaluated for the 12 IBPGR minimum taxonomic characters. An additional nine phenotypic characters and six biochemical markers were evaluated to enable diversity comparisons. Cluster analysis of the various sets of data revealed groups of accessions for each of the three data sets. A poor level of agreement (congruence) between data sets was observed in all comparisons indicating that, for cultivated barley at least, diverse collections according to the IBPGR minimum descriptors is not necessarily related to equivalent levels of diversity in other genetic characters. Implications of the relevance of the IBPGR descriptor list and appropriate collection strategies are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222331
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    Paper (German National Licenses)
    Fulltext
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