ISSN:
1420-9071
Keywords:
Arylsulfatase A
;
natural substrate
;
ascorbate-2-sulfate
;
HPLC-amperometric detection
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Summary Arylsulfatase activities in biological materials are too low to be detected by the methods available hitherto. A sensitive and specific assay method for arylsulfatase A (AS-A) has been developed in the present study. Ascorbate-2-sulfate is known to be a specific natural substrate of AS-A; the ascorbic acid liberated by the action of AS-A was quantitatively determined using HPLC equipped with an amperometric detector. The method was used to analyze the activity of AS-A in biological materials.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01975881
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