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  • Life and Medical Sciences  (8)
  • B chromosomes  (5)
  • tomato  (5)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Genetica 97 (1996), S. 65-72 
    ISSN: 1573-6857
    Keywords: Secale cereale ; rye ; B chromosomes ; inbred lines ; pairing ; pollen mitosis ; transmission genotypes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract B chromosomes from an experimental population of the Japanese JNK strain of rye, isogenic for its Bs, have been backcrossed into twelve different inbred lines. The experiment is a way of studying the effects of the Bs against a range of different homozygous A chromosome backgrounds. This publication deals with pairing effects of both the As and the Bs, and their interactions, and with pollen mitosis. At meiosis there is a genotypic component to B effects, and they do not appear to act solely through a physical disturbance within the nucleus. In pollen the Bs are always present in more than 50% of the grains regardless of their pairing behaviour during meiosis; this result fits with a ‘parasitic’ model of the activity of rye Bs.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1573-6849
    Keywords: B chromosomes ; genomicin situ hybridization ; nuclear architecture ; Secale cereale ; Triticum aestivum cv ; Lindström
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The B chromosomes (Bs) of rye (Secale cereale) have been studied at interphase in terms of their chromatin organization, patterns of nuclear disposition and physical association in plants with two, three, and four Bs. The study was made in the Lindström strain of hexaploid wheat, which carries the rye Bs as an addition line, byin situ hybridization with a B-specific probe and by genomicin situ hybridization (GISH) with rye genomic DNA, enabling whole chromosome painting. Repetive sequences common to the As and Bs of rye allow for visualization of the rye B at interphase in the wheat background. A B-specific probe enables the orientation of two or more Bs to be determined, and the combination of both probes used together gives information on the disposition of the Bs and on their patterns of physical association within the nucleus. The Bs form linear ‘strings’, and the ends of their long arms, which can be detected by the B-specific probe, are usually located within the hemisphere of the nucleus that has the least condensed chromatin. There is dose-dependent association, and even numbers (2B, 4B) have a greater preference for association than odd ones (3B).
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1573-6849
    Keywords: B chromosomes ; interphase chromatin ; rDNA organization ; rye
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Patterns of rye rDNA organization in interphase nuclei were studied through the use ofin situ hybridization in spreads of root meristem cells from plants with and without B chromosomes (Bs). In cells from plants without Bs each rDNA locus is organized as a single perinucleolar knob of condensed chromatin with decondensed chromatin inside the nucleolus. In plants with Bs there is a marked modification of the pattern, found in more than 23% of nuclei, which involves several regions of condensed chromatin interspersed with decondensed chromatin inside the nucleolus. This B-induced alteration in rDNA interphase organization suggests a change in expression of the rRNA genes located on the A chromosomes probably related to the reduction in nuclear RNA observed previously in plants with Bs. The influence of the Bs on the expression of A chromosome genes, through rearrangement of interphase chromatin, could provide the basis of an explanation for some of the known phenotypic effects of B chromosomes in rye.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Euphytica 32 (1983), S. 845-855 
    ISSN: 1573-5060
    Keywords: Lycopersicon esculentum ; tomato ; flavor ; sensory evaluation ; soluble solids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Advanced high sugar and acid breeding lines of tomatoes (Lycopersion esculentum Mill.) were rated higher in sweetness, sourness and overall flavor intensity than the standard cultivars Cal Ace or T3. Titratable acidity and soluble solids content were major contributors to differences in overall flavor intensity. The results demonstrate that significant improvement in tomato flavor can be attained by increasing sugar and acid contents in tomato fruits by genetic manipulation. Current evidence indicates that breeding for high soluble solids in horticulturally acceptable tomato cultivars is justified.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Euphytica 35 (1986), S. 575-582 
    ISSN: 1573-5060
    Keywords: Lycopersicon ; tomato ; salinity ; germination ; germplasm ; breeding ; salt-tolerance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The potential to improve seed germination responses to salinity was evaluated for 13 accessions representing six wild Lycopersicon species and 20 accessions of L. esculentum. Germination response times increased in all accessions at 100 mM NaCl. Analysis indicated that one accession of L. peruvianum (PI126435) germinated faster under high salinity than all other accessions and was closely followed by L. pennellii (LA716). The fastest germinating L. esculentum accession, PI174263, ranked third. Additional wild ecotypes exhibiting rapid germination at 100 mM NaCl were identified among L. pimpinellifolium and L. peruvianum.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1573-5060
    Keywords: Lycopersicon esculentum ; tomato ; cold tolerance ; seed germination ; genetic analysis ; missing data ; response-time data
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary In studies to determine the inheritance of response-time traits, such as time to seed germination, some viable individuals may fail to respond during an experiment. If these right-censored observations are ignored, sample means and variances will be underestimated. This is illustrated using data from time to seed germination at 9°C for Lycopersicon esculentum (Mill.) fast germinating PI 120256, slow-germinating T3 and their reciprocal F1, F2 and backcross progeny. This paper presents methods to detect and to accommodate right-censored data in generation means analysis. Genetic interpretations derived from corrected and uncorrected estimates of generation means and variances are compared. Correction for right-censoring increased estimates of environmental and phenotypic variances, and decreased heritability estimates.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Euphytica 31 (1982), S. 869-883 
    ISSN: 1573-5060
    Keywords: Lycopersicon ; tomato ; low temperature germination ; survival analysis ; high altitude ecotypes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Low temperature germination responses were evaluated for 18 high altitude accessions representing five wild Lycopersicon species and 19 accessions of L. esculentum which have reputed ability to germinate in the cold. Survival analysis indicated that one accession of L. chilense germinates better at 10°C than PI 120256, the fastest-germinating L. esculentum genotype, and that PI 120256 germinates as well as PI 126435 (L. peruvianum). Additional wild ecotypes exhibiting rapid germination at 10°C were identified from L. peruvianum and L. hirsutum. These ecotypes may possess genetic potential for introgressing cold germination ability into L. esculentum cultivars.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Euphytica 40 (1989), S. 187-191 
    ISSN: 1573-5060
    Keywords: Lycopersicon esculentum ; tomato ; Pyrenochaeta lycopersici ; corky root ; brown root rot ; resistance ; greenhouse screening method
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A greenhouse screening method for corky root (Pyrenochaeta lycopersici) resistance in the tomato (Lycopersicon esculentum) is described in detail. In determining the reliability of the method, known resistance sources within wild asccessions, commerical cultivars and breeding lines were evaluated for resistance in fields naturally infested with corky root and their response compared under greenhouse conditions in soil heavily infested with corky root and artifically maintained at temperatures between 10–13°C. The procedure developed can be used year round and improves the efficiency of recovering resistant progeny in large segregrating populations.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Molecular Reproduction and Development 33 (1992), S. 108-115 
    ISSN: 1040-452X
    Keywords: Zona binding proteins ; Seminal plasma ; Fertilization ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: A group of low Mr (16 kDa - 23 kDa) glycoproteins on ejaculated boar spermatozoa have been shown to have high affinity for homologous zona pellucida glycoproteins (ZPGPs). These ZPGP binding proteins are derived from seminal plasma as shown by their absence from epididymal spermatozoa and their presence in seminal plasma as identified by N-terminal amino acid sequence analysis. They bind to ZPGPs by a polysulphate recognition mechanism similar to that found for proacrosin-ZPGP interactions. The haemagglutination activity of boar seminal plasma is also associated with these low Mr glycoproteins. It is suggested that they play a role in regulating the rate of sperm capacitation and survival in the female reproductive tract. © 1992 Wiley-Liss, Inc.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Molecular Reproduction and Development 38 (1994), S. 77-84 
    ISSN: 1040-452X
    Keywords: Cauda epididymidis ; Sperm activation ; Calcium ions ; Guanylate cyclase ; Adenylate cyclase ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The role of intracellular signal transduction mechanisms in regulating the motility and metabolism of rat spermatozoa in undiluted caudal epididymal fluid (CEF) was examined. Samples of CEF containing immotile spermatozoa were exposed to drugs and other agents that either stimulate signal transduction pathways or mimic the action of their second messengers. Under these conditions, sperm motility in 25-30 nl of CEF was stimulated by calcium ions (Ca2+), N,2′ -O-dibutyryl-guanosine 3′:5′ -cyclic monophosphate (dibutryl cGMP), cyclic adenosine 3′:5′-monophosphate (cAMP), N6,2′-O-dibutyryladenosine 3′:5′ -cyclic monophosphate (dibutyryl cAMP), 8-bromoadenosine 3′:5′ -cyclic monophosphate (8-bromo cAMP), caffeine, theophylline and bicarbonate ions (HCO3-). Other agents such as magnesium ions (Mg2+), veratridine, phospholipase C (PLC), ionophore A23187, 1,2-dioctenoyl-sn-glycerol (DAG), phorbol 12-myristate 13-acetate, phospholipase A2 (PLA2), arachidonic acid, and melittin did not significantly influence motility. In the presence of radiolabelled energy substrates, untreated (immotile) spermatozoa in samples of CEF utilised D-[U-14C]glucose and [1-14C]acetate as exogenous energy sources for oxidative metabolism. No detectable 14C-lactate was produced, and none of the drugs altered the rate of glycolytic or oxidative metabolism. The findings suggest that the motility of rat caudal epididymal spermatozoa is regulated by Ca2+ and the guanylate cyclase and adenylate cyclase pathways, but not through the PLC and PLA2 pathways. Also, their metabolism of exogenous substrate was uncoupled from the induction of motility, and their oxidative capacity exceeded the rate of flux of glucose-carbon through the glycolytic pathway. © 1994 Wiley-Liss, Inc.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
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