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  • 1
    Electronic Resource
    Electronic Resource
    Bioaccumulation of pollutants and changes in population parameters in the gastropod mollusc Austrocochlea constricta (1994)
    Springer
    Archives of environmental contamination and toxicology 26 (1994), S. 367-373 
    Details
    ISSN: 1432-0703
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Medicine
    Notes: Abstract The gastropod mollusc Austrocochlea constricta was collected from four marine locations within the Newcastle region of New South Wales, Australia to assess the range and distribution of major bioaccumulated organic and heavy metal pollutants. The metals and organopollutants were extracted from the soft tissue and the shell of the mollusc for analysis using atomic absorption spectroscopy (AAS) and gas chromatography-mass spectroscopy (GC-MS). The organisms accumulated heavy metals from the sea water and concentrated them to levels substantially higher than those in the surrounding environment. A wide range of organopollutants was also detected in varying levels in Austrochochlea from each location. Abietic and dehydroabietic acids were detected only in the shell and not in the soft tissue suggesting that the shell may act as a “toxic waste sink” to facilitate the removal of potentially harmful compounds from the more metabolically active soft tissue. Aliphatic hydrocarbon contaminants were detected in Austrocochlea derived from the three sites associated with either heavy industry or recreational boating, but no hydrocarbons were detected in organisms from the control site. It was concluded that Austrocochlea may serve as a useful biomonitoring system of pollutants bioaccumulated from marine environments.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00203564
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  • 2
    Electronic Resource
    Electronic Resource
    Differential bioaccumulation of heavy metals and organopollutants in the soft tissue and shell of the marine Gastropod, Austrocochlea constricta (1995)
    Springer
    Archives of environmental contamination and toxicology 28 (1995), S. 35-39 
    Details
    ISSN: 1432-0703
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Medicine
    Notes: Abstract Austrocochlea constricta (Gastropodia) has the potential to act as a useful biomonitoring system of pollutants in the marine environment. The organism was used in the present study to investigate the distribution of both heavy metals and organopollutants in the soft tissue and shell of individuals sampled from various sites in the Newcastle region of New South Wales (Australia). The heavy metals and organopollutants were extracted from the mollusc and determined by atomic absorption spectroscopy (AAS) and gas chromatography-mass spectrometry (GC-MS), respectively. The heavy metals zinc, cadmium, copper, and lead were detected both within the soft tissue and the shell extract, but the proportions of these metals partitioned into the extracts were different for each location. It was concluded that the accurate evaluation of heavy metal exposure in Austrocochlea should include analyses of both soft tissue and shell fractions. The accumulation of aliphatic hydrocarbons was more rigorously partitioned between the two tissue types than the heavy metals. Short chain aliphatic hydrocarbons were limited to the soft tissue while the longer chain aliphatic hydrocarbons were primarily confined to the shell. The data suggest that the shell may act as a safe storage matrix for toxic contaminants resistant to soft tissue detoxification mechanisms. The organic contaminants measured in the shell can be utilized as a valuable indicator of refuse dumping or release of pulp mill effluent as well as petrochemical contamination from industrial and recreational activity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00213966
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Deciduogenic effects of mediators of the polyphosphatidylinositol pathway in pseudopregnant mice (1992)
    Springer
    Cellular and molecular life sciences 48 (1992), S. 741-745 
    Details
    ISSN: 1420-9071
    Keywords: Decidualization ; pseudopregnancy ; uterine biochemistry ; polyphosphatidylinositol pathway ; concanavalin A ; calcium ionophore ; phorbol ester
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Intraluminal injections (15 μl) of either concanavalin A (125 μg) or ionophore A 23187 (0.01 μmol) induced a decidual cell reaction (DCR) in the uterus of day 4.5 pseudopregnant mice. However, when these agents were administered in different combinations with each other or with CaCl2 (15 μmol) and phorbol-12-myristate-13-acetate (1.6 nmol), interacting effects occurred to either enhance or inhibit each of the others' independent deciduogenic capacities. The results suggest that the polyphosphatidylinositol pathway and Ca2+ are involved in the induction of the DCR in mice with complex interactions occurring between the active components of the pathway to modulate the outcome of the transformation process.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02124291
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  • 4
    Electronic Resource
    Electronic Resource
    Effect of decapacitation factor on the oxygen uptake of rabbit spermatozoa recovered from the uterus (1975)
    Springer
    Cellular and molecular life sciences 31 (1975), S. 80-81 
    Details
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Résumé L'addition d'un facteur de décapacitation préparé à partir du plasma séminal de lapin et de taureau a généralement produit une augmentation supplémentaire de l'absorption d'oxygène, quand il a été ajouté à la suspension de spermatozoïdes enlevée de l'utérus. Le facteur de décapacitation n'a exercé aucun effet stimulant similaire sur les spermatozoïdes de lapin avant l'incubation dans l'utérus et n'a montré lui-même aucune absorption d'oxygène appréciable.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01924689
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  • 5
    Electronic Resource
    Electronic Resource
    Intracellular signal transduction mechanisms of rat epididymal spermatozoa and their relationship to motility and metabolism (1994)
    New York, NY [u.a.] : Wiley-Blackwell
    Molecular Reproduction and Development 38 (1994), S. 77-84 
    Details
    ISSN: 1040-452X
    Keywords: Cauda epididymidis ; Sperm activation ; Calcium ions ; Guanylate cyclase ; Adenylate cyclase ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The role of intracellular signal transduction mechanisms in regulating the motility and metabolism of rat spermatozoa in undiluted caudal epididymal fluid (CEF) was examined. Samples of CEF containing immotile spermatozoa were exposed to drugs and other agents that either stimulate signal transduction pathways or mimic the action of their second messengers. Under these conditions, sperm motility in 25-30 nl of CEF was stimulated by calcium ions (Ca2+), N,2′ -O-dibutyryl-guanosine 3′:5′ -cyclic monophosphate (dibutryl cGMP), cyclic adenosine 3′:5′-monophosphate (cAMP), N6,2′-O-dibutyryladenosine 3′:5′ -cyclic monophosphate (dibutyryl cAMP), 8-bromoadenosine 3′:5′ -cyclic monophosphate (8-bromo cAMP), caffeine, theophylline and bicarbonate ions (HCO3-). Other agents such as magnesium ions (Mg2+), veratridine, phospholipase C (PLC), ionophore A23187, 1,2-dioctenoyl-sn-glycerol (DAG), phorbol 12-myristate 13-acetate, phospholipase A2 (PLA2), arachidonic acid, and melittin did not significantly influence motility. In the presence of radiolabelled energy substrates, untreated (immotile) spermatozoa in samples of CEF utilised D-[U-14C]glucose and [1-14C]acetate as exogenous energy sources for oxidative metabolism. No detectable 14C-lactate was produced, and none of the drugs altered the rate of glycolytic or oxidative metabolism. The findings suggest that the motility of rat caudal epididymal spermatozoa is regulated by Ca2+ and the guanylate cyclase and adenylate cyclase pathways, but not through the PLC and PLA2 pathways. Also, their metabolism of exogenous substrate was uncoupled from the induction of motility, and their oxidative capacity exceeded the rate of flux of glucose-carbon through the glycolytic pathway. © 1994 Wiley-Liss, Inc.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrd.1080380113
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    Paper (German National Licenses)
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