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Organization of the projections from barrel cortex to thalamus in mice studied with Phaseolus vulgaris-leucoagglutinin and HRP (1987)

Hoogland, P. V. ; Welker, E. ; Loos, H.

Springer

Experimental brain research 68 (1987), S. 73-87

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ISSN: 1432-1106

Keywords: Barrel ; Barreloid ; Thalamus ; Somatosensory system ; Mouse ; Phaseolus vulgaris ; HRP

Source: Springer Online Journal Archives 1860-2000

Topics: Medicine

Notes: Summary In order to elucidate the geometric organization of projections from the barrel cortex to the thalamus, iontophoretic injections of the anterograde tracer Phaseolus vulgaris-leucoagglutinin were made. The injections were confined to one barrel column (i.e. barrel in layer IV + cortical tissue above and below it). Axonal terminations could be demonstrated in three thalamic nuclei: reticularis (RT), ventrobasalis (VB) and posterior (PO). Anterograde terminal labelling was obtained in RT + VB; in PO only; or in RT + VB + PO. The terminals labelled in PO were much larger than those in RT and VB. The termination areas in RT, VB and PO were shaped like rods which have a rostro-caudal orientation. These cortico-thalamic projections are discretely and topographically organized. The clearest such arrangement was found in VB. Here, projections from the A row of barrels in BF terminate dorsally, whereas those from the C row end ventrally. Barrel A1 projects to the lateral part of VB, whereas A4, to more medial parts; other rows are arranged similarly. These results were compared with the distribution of thalamo-cortical projection neurons that were labelled after iontophoretic HRP injections in individual barrels. We concluded that the corticothalamic projections originating from one barrel column contact an arc of barreloids in VB.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00255235

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A model system for a fluorometric biosensor using permeabilized Zymomonas mobilis or enzymes with protein confined dinucleotides (1993)

Thordsen, O. ; Lee, S. J. ; Degelau, A. ; [et al.]

New York, NY [u.a.] : Wiley-Blackwell

Biotechnology and Bioengineering 42 (1993), S. 387-393

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ISSN: 0006-3592

Keywords: biosensor ; glucose ; fructose ; gluconolactone ; sorbitol ; Zymomonas mobilis ; permeabilization ; NADPH ; fluorescence ; bioprocess monitoring ; Chemistry ; Biochemistry and Biotechnology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology

Notes: Using permeabilized Zymomonas mobilis or glucose-fructose oxidoreductase isolated from this microorganism a model system for biosensors with a protein confined NADP(H) cofactor for the determination of glucose, fructose, gluconolactone, and sorbitol was developed. Either permeabilized microorganisms containing the oxidoreductase or the pure enzyme were confined via membrane separation in a small measuring chamber, that was integrated into a flow injection analysis system (FIA). The measuring principle was the monitoring of the NAD(P)H fluorescence, excited at 360 nm and measured at 450 nm. NADP(H), which is confined in the protein complex, was oxidized or reduced during the enzymatic reactions and the changes in the fluorescence intensity were related to the substrate concentration. The sensitivity of the system covered a range from 0.001 to 100 g/L of the analyte depending on substrate and operating conditions. The applicability of this model system for bioprocess monitoring was proved using samples from a Pseudomonas pseudoflava cultivation. © 1993 John Wiley & Sons, Inc.

Additional Material: 13 Ill.