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  • 1
    Electronic Resource
    Electronic Resource
    The effect of monensin on intracellular transport and secretion of α-amylase isoenzymes in barley aleurone (1986)
    Springer
    Planta 167 (1986), S. 252-259 
    Details
    ISSN: 1432-2048
    Keywords: Aleurone (enzyme secretion) ; α-Amylase ; Gibberellin and enzyme secretion ; Hordeum (enzymes) ; Monensin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The effect of monensin on the secretion of α-amylase and other enzymes from the aleurone layer of barley (Hordeum vulgare L. cv. Himalaya) was studied by electrophoresis followed by fluorography and by pulse-chase and organelle-isolation experiments. Monensin markedly inhibits the secretion, but not the synthesis, of α-amylase, acid phosphatase, and at least four other proteins from the aleurone layer. Monensin treatment causes α-amylase to accumulate within the protoplast, but its effect on the different α-amylase isoenzymes is not equal. The accumulation of isoenzyme 2 is not influenced by monensin while isoenzymes 1, 3 and 4 are not secreted but rather accumulate in the cell when monensin is included in the incubation medium. The α-amylase and acid-phosphatase activities which accumulate within the aleurone cells following treatment with monensin are localized in an organelle having a buoyant density greater than that of endoplasmic reticulum and less than that of mitochondria. In pulse-chase experiments with [35S]methionine, labelled proteins accumulate in this organelle in the presence of monensin and do not appear in the incubation medium. We conclude that monensin inhibits the secretion of proteins from the barley aleurone layer by influencing their intracellular transport.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00391423
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Monensin inhibits the secretion of α-amylase but not polysaccharide slime from seedling tissues ofZea mays (1988)
    Springer
    Protoplasma 142 (1988), S. 36-45 
    Details
    ISSN: 1615-6102
    Keywords: α-Amylase ; Corn (Zea mays) seedlings ; Growth ; Monensin ; Polysaccharide slime ; Secretion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The effect of monensin on polysaccharide slime secretion by root tips of corn (Zea mays) was studied. Various treatment times and ionophore concentrations were tested: none resulted in inhibition of slime secretion. Because monensin changes the pH of the medium, its effect was also monitored in strongly buffered media and at different pH's. Even in such media, monensin did not inhibit slime secretion. We also measured the effect of the drug after a pulse with [3H]fucose or a pulse followed by a chase. The amount of labeled slimed secreted was not altered by the ionophore. However, 10μM monensin affected the development of root tips and drastically reduced their growth. We showed that monensin inhibits the secretion of α-amylase by the scutellum of the same plantlet. The importance of the nature of the secretory compound in relation to monensin inhibition of its secretion is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01273224
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    The enhancement of acidity in non-aqueous solvents (1973)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 264 (1973), S. 381-385 
    Details
    ISSN: 1618-2650
    Keywords: Acidität ; Steigerung durch Erdalkalisalze in organ. Lösungsmitteln ; Best. von Organ. Säuren ; Volumetrie ; Salzeffekt in organ. Lösungsmitteln
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Summary The acid strength of short-chain carboxylic acids in acetonitrile and acrylonitrile can, by addition of anhydrous alkaline earth perchlorates, be enhanced sufficiently to permit titration of the acid with a tertiary amine in the presence of acid anhydride. This salt effect has been studied by means of potentiometric and automatic thermometric titrations and two distinct mechanisms are thought to be involved: a metathetical reaction at low salt concentrations and a proton desolvation effect at high salt concentrations. High-resolution infra-red spectroscopy has been used to assess the extent of sol vent-cation interaction in acetonitrile. Several equations are used to describe the probable interactions taking place in such salt enhancement phenomena.
    Notes: Zusammenfassung Der Säuregrad von kurzkettigen Carbonsäuren in Acetonitril und Acrylnitril kann durch Hinzufügen von wasserfreien Perchloraten der Erdalkalien genügend gesteigert werden, um bei Vorhandensein von Säureanhydrid eine Titration der. Säure mit einem tertiären Amin zu ermöglichen. Dieser Salzeffekt wurde mit Hilfe von potentiometrischen und automatischen thermometrischen Titrationen untersucht, und es wird angenommen, daß dabei zwei verschiedene Vorgänge eine Rolle spielen: eine Austauschreaktion bei schwachen und ein Desolvatationseffekt bei hohen Salzkonzentrationen. Zur Beurteilung des Ausmaßes der Wechselwirkung zwischen Lösung und Kation in Acetonitril wurde die Infrarot-Spektroskopie mit hoher Auflösung benutzt. Die wahrscheinlich stattfindenden Reaktionsvorgänge werden anhand verschiedener Gleichungen beschrieben.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00423638
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    Paper (German National Licenses)
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