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Digitale Medien

Effects of Okadaic Acid on Rat Colon (1998)

Hosokawa, Masaya ; Tsukada, Hideaki ; Saitou, Tatsuhiko ; [weitere]

Springer

Digestive diseases and sciences 43 (1998), S. 2526-2535

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ISSN: 1573-2568

Schlagwort(e): OKADAIC ACID ; MICROTHROMBUS ; COLON ; PARACELLULAR PATHWAY

Quelle: Springer Online Journal Archives 1860-2000

Thema: Medizin

Notizen: Abstract Effects of okadaic acid (OA) on mucosal damagewere examined in rat colon. OA was sprinkled on ratcolon mucosa under observation with anelectronic-endoscopic system, and OA was also applied tothe in vivo microscopic field. The OA-induced changesin transepithelialconductance (Gt) weremeasured by the Ussing voltage clamp technique. Byendoscopic observation, the luminal sprinkling of OA (60nmol/kg) evoked transient microthrombi in the submucosalvenule, which was followed by mucosal edema.Histological study after endoscopic observation showedsubmucosal fluid retention, suggesting an increase of vascular permeability. The microthrombi werealso detected by in vivo microscopy. Byelectrophysiological study after endoscopic observationwith and without OA addition, the basal Gtvalues were 54 ± 6.2 and 36.2 ± 4.2 mS/cm2,respectively (P 〈 0.01). Furthermore in control rats,the serosal addition of OA evoked an increase inGt in a concentration-dependent mannerwithout increasing lactate dehydrogenase release. 2,4,6-Triaminopyrimidinium inhibitedOA-induced Gt change by 60%. These resultsindicate that OA evokes an increase in paracellularpermeability of epithelium. We conclude that thedeveloped microthrombi are the first key event of OA-induced mucosaldamage, followed by an increase in permeability in thesubmucosal venule and in the paracellular pathway of theepithelium.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1023/A:1026658921369

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Digitale Medien

Chemical separation methods for common microbes (1961)

Nakamura, Hiroshi

New York, NY [u.a.] : Wiley-Blackwell

Journal of Biochemical and Microbiological Technology and Engineering 3 (1961), S. 395-403

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ISSN: 0368-1467

Schlagwort(e): Chemistry ; Biochemistry and Biotechnology

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung

Notizen: To collect microbes from suspensions by a process other than centrifugation, it has been found effective to add inorganic and organic electrolytes in the colloidal state. The addition of electrolytes causes a neutralization of charges on the particles in suspension resulting in their coagulation and precipitation. High-molecular-weight compounds which form micelles also promote coagulation and precipitation. It is believed that chemical collection is an extremely efficient method of treatment for the mass production of microbial suspensions.