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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Biology and fertility of soils 5 (1987), S. 120-125 
    ISSN: 1432-0789
    Keywords: Fungal melanins ; Humic acid ; Infrared analysis ; Free radicals
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Humic acid-type polymers (melanins) synthesized in culture media by the fungi Aspergillus glaucus, Eurotium echinulatum, Hendersonula toruloidea, Stachybotrys atra and Aspergillus sydowi were analysed for elemental composition, functional group content, infrared (IR) and electron spin resonance (ESR) properties. Results were discussed in comparison with range values referred for soil humic acids. The fungal polymers showed significant differences in carboxyl and nitrogen content and C/H atomic ratios, reflecting a different degree of condensation (aromaticity) among the various samples. IR analysis gave evidence of: (a) the predominant aromatic character of melanins from A. glaucus, E. echinulatum and H. toruloidea; (b) the high content of aliphatic and olefinic components of S. atra melanin; (c) the typical presence of amide bonds in the nitrogen-richest melanins from A. sydowi and H. toruloidea; and (d) the generally low amount of free carboxyl groups, which often appeared involved in hydrogen bonds. ESR spectra showed that all the melanins studied contained appreciable concentrations of organic free radicals of prevailing semiquinonic nature and of the same order of magnitude commonly measured in humic acids from soil and other sources. The free electron concentration was shown to be directly related to the C/H atomic ratio and to the degree of aromaticity shown by IR analysis. This indicated that the highest free radical content in the melanins from E. echinulatum and A. glaucus was associated with the highest presence of condensed aromatic structures. Humic acid-type polymers synthesized by soil fungi may, therefore, contribute to the total free radical content of soil humic substances and play important roles in all reactions involving free radicals in soils and related environments.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1573-5036
    Keywords: Biodegradation ; Biomass ; Carbon ; Cornstalks ; Humus formation ; Wheat straw
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The effect of soil type and incubation period on the biodegradation, incorporation into biomass, and stabilization in humus of14C-labeled cornstalk and/or wheat straw lignin, polysaccharide, and protein fractions were followed for one year. After 6 months, 56–68%, 6–21%, 71–81%, 63–75%, and 56–68% from wheat straw and from the lignin, polysaccharide, and protein fraction of wheat straw had been lost as CO2, respectively. Loss of CO2 increased only slightly with further incubation. Greater amounts of CO2, especially during the early incubation stages, were evolved from neutral and alkaline soils (pH 7.0, 7.4, 7.8) than from acid soils (pH 5.0, 5.5). After one year, a major portion of the residual C from lignin was recovered in the humic acid fraction, relatively small amounts, 5 to 17%, were lost upon acid hydrolysis, and generally 〈1% was found present in the biomass. Lesser amounts of the polysaccharide and protein carbons were incorporated into the humic acid, 17–20% and 16–27%, respectively. Relatively greater amounts of the residual carbons of the polysaccharide and protein were incorporated into the biomass, 4.9–7.8% and 4.6–13.4%, respectively and higher percentages were lost upon acid hydrolysis, 56 to 81%. The results for the whole wheat straw were very similar to those of the protein fraction. Overall, more residual C was stabilized into humic acid in the acid soils than in the neutral soils.
    Type of Medium: Electronic Resource
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