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  • 1
    Electronic Resource
    Electronic Resource
    Photodynamic therapy of bladder cancer —uptake and phototoxicity of photosan in vitro (1991)
    Springer
    Urological research 19 (1991), S. 353-356 
    Details
    ISSN: 1434-0879
    Keywords: Bladder cancer ; Electron microscopy ; Photodynamic therapy ; Photosan ; Phototoxicity ; Tumor selectivity ; Video fluorescence microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The uptake of photosan and the intracellular sites of photoradiation-induced damage were investigated in vitro in bladder carcinoma cells and in normal bladder cells. Cells were examined by phase contrast, fluorescence and electron microscopy. The concentration of photosan, measured in μg/106 cells, showed a good correlation to the incubation time. At all incubation times, control cells showed a lower uptake when compared with tumor cells. Following photodynamic therapy (PDT), phase-contrast microscopy revealed marked changes in tumor cells, whereas only minor effects could be detected at the cell membrane of the control cells. Following PDT, most of the investigated cells showed onanges of the mitochondria and cytoplasma. These changes consisted of dissolution of the cristae, predominantly in the central part of the mitochondria. Twenty-four hours after PDT the shape of the mitochondria had changed markedly and the cristae were found to be completely destroyed. Moreover, the cystoplasma showed numerous vacuoles, and the number of mitochondria was decreased compared to non-treated cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00310149
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Aminolevulinic acid for photodynamic therapy of bladder carcinoma cells (1996)
    Springer
    Urological research 24 (1996), S. 285-289 
    Details
    ISSN: 1434-0879
    Keywords: Bladder cancer ; Photodynamic therapy ; Aminolevulinic acid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract A new concept in photosensitizing tumor cells is photosensitizer synthesis in situ. Aminolevulinic acid (ALA) is a precursor of protoporphyrin IX (PP IX), a potent photosensitizer. The goal of our study was to examine dark toxicity, phototoxic potential, metabolism of ALA and morphological alterations in Waf bladder cancer cells. Dark toxicity of Waf cells was observed after incubation with ALA, beginning at a concentration of 15 mM. Photodynamic treatment with ALA at concentrations of 1,5 and 10 mM showed a drug- and light-dose-dependent cell survival rate in comparison to a control group. Two incubation times of 3.5 and 5.5 h were compared for cell survival. After a longer incubation time of 5.5 h, cell survival was decreased in all experiments; this is consistent with our extraction data where higher fluorescence was found after 5.5 than after 3.5 h. The results show that ALA-induced photosensitization has a high potential for photodynamic therapy (PDT) of superficial bladder carcinoma.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00304778
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Comparison of two routes of photosensitizer administration for photodynamic therapy of bladder cancer (1994)
    Springer
    Urological research 22 (1994), S. 21-23 
    Details
    ISSN: 1434-0879
    Keywords: Bladder cancer ; Photodynamic therapy ; Intravenous injection ; Intravesical instillation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Photodynamic therapy (PDT) consists in administration of a photosensitizer and subsequent irradiation of the tumor with visible light. Routinely the photosensitizer is given intravenously (i.v.). The goal of our study was to examine whether intravesical (i.b.) instillation of the photosensitizer for PDT of bladder cancer might be feasible. Therefore, the uptake of chlor-aluminum-sulfonated phthalocyanine (CASPc) in bladder, bladder tumor, skin, and muscle in a rat bladder cancer model after i.v. injection and i.b instillation was compared. The efficacy of PDT after either method of administration was also evaluated. The CASPc concentration in bladder tumor after i.v. injection was approximately 1.5-fold that after i.b. instillation. The ratio of CASPc concentration between bladder tumor and normal bladder was approximately 2:1 after administration by either route. There was no systemic absorption of CASPc after i.b. instillation; hence no systemic side effects are expected. PDT showed similar effects on bladder tumor after either method of administration, but less side effects on normal bladder wall after i.b instillation. Our results demonstrate that i.b. instillation of CASP for PDT of superficial bladder cancer seems to have advantages over i.v. injection.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00431544
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    Paper (German National Licenses)
    Fulltext
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