ISSN:
1432-0878
Keywords:
Key words: Intracellular collagen-like material
;
Blastemal cells
;
Cytokines
;
Organoid cultures
;
Mouse
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Abstract. Blastemal cells of embryonic mouse limb buds (day 12) were cultivated in organoid cultures in the presence of the human recombinant cytokines interleukin-1β (IL-1β) and tumour necrosis factor α (TNF-α). The effects of both cytokines (applied alone or together) on mesenchymal cells were demonstrated by electron microscopy. Cultures treated with TNF-α (alone or in combination with IL-1β) showed several mesenchymal cells with numerous irregularly shaped membrane-bordered cavities containing thick bundled tannic-acid-positive fibrillar structures that resembled loosened collagen fibrils, whereas cells exposed to IL-1β alone did not exhibit such changes. These findings are discussed in the light of two hypotheses: the phagocytosis of extracellular collagen fibrils, and fibrillogenesis resulting from incongruity of synthesis and secretion rates of procollagen; our results favour the former.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s004410050613
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