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  • 1
    Digitale Medien
    Digitale Medien
    Intracellular collagen-like material in mouse embryonic mesenchymal cells incubated with TNF-α and IL-1β in organoid culture (1996)
    Springer
    Cell & tissue research 284 (1996), S. 509-515 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Key words: Intracellular collagen-like material ; Blastemal cells ; Cytokines ; Organoid cultures ; Mouse
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Abstract. Blastemal cells of embryonic mouse limb buds (day 12) were cultivated in organoid cultures in the presence of the human recombinant cytokines interleukin-1β (IL-1β) and tumour necrosis factor α (TNF-α). The effects of both cytokines (applied alone or together) on mesenchymal cells were demonstrated by electron microscopy. Cultures treated with TNF-α (alone or in combination with IL-1β) showed several mesenchymal cells with numerous irregularly shaped membrane-bordered cavities containing thick bundled tannic-acid-positive fibrillar structures that resembled loosened collagen fibrils, whereas cells exposed to IL-1β alone did not exhibit such changes. These findings are discussed in the light of two hypotheses: the phagocytosis of extracellular collagen fibrils, and fibrillogenesis resulting from incongruity of synthesis and secretion rates of procollagen; our results favour the former.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s004410050613
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  • 2
    Digitale Medien
    Digitale Medien
    Negative metabolic effects of cyclic GMP are altered in renal hypertension induced cardiac hypertrophy (1997)
    Springer
    Basic research in cardiology 92 (1997), S. 8-16 
    Details
    ISSN: 1435-1803
    Schlagwort(e): Renal hypertension ; cardiac hypertrophy ; cyclic GMP ; myocardial O2 consumption ; rabbit
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract We tested the hypothesis that increasing myocardial cyclic GMP levels would reduce myocardial O2 consumption and that renal hypertension (One Kidney-One Clip, 1K1C)-induced cardiac hypertrophy would change this relationship. Four groups of anesthetized open-chest New Zealand white rabbits (N=26) were utilized. Either vehicle or 3-morpholinosydnonimine (SIN-1) (10−4M, a guanylate cyclase activator) was topically applied to the left ventricular surface of control or 1K1C rabbits. Coronary blood flow (radioactive microspheres) and O2 extraction (microspectrophotometry) were used to determine O2 consumption. Myocardial cyclic GMP levels were determined by radioimmunoassay. Guanylate cyclase activity was measured by conversion of GTP to cyclic GMP. 1K1C rabbits had a greater heart weight-to-body weight ratio (3.29±0.15) than controls (2.63±0.19). Systolic blood pressure was higher in 1K1C rabbits than in controls. In control rabbits, cyclic GMP levels (pmoles/g) were higher in SIN-1-treated (EPI: 7.5±1.6; ENDO: 8.1±1.5) than in vehicle-treated animals (EPI: 5.4±0.4; ENDO: 5.6±0.6). This effect was enhanced in 1K1C rabbits, with cyclic GMP levels in the SIN-1-treated group (EPI: 11.9 ±1.3; ENDO: 13.0±1.5) almost double those observed in the vehicletreated group (EPI: 6.3±0.8; ENDO: 7.7±1.1). There were no significant differences in basal or maximally stimulated guanylate cyclase activity between controls and 1K1C rabbits. Myocardial O2 consumption (ml O2/min/100 g) was significantly less in the EPI region of control animals treated with SIN-1 (7.2±1.2) than in the same region of controls treated with vehicle (9.1±2.0). Myocardial O2 consumption was also significantly less in SIN-1-than vehicle-treated 1K1C animals (SIN-1-treated: EPI: 6.9±0.8; ENDO: 6.2±0.7; vehicle-treated: EPI: 10.0±0.8; ENDO: 12.5±3.0). There was no significant difference in O2 consumption between control and 1K1C animals after treatment with SIN-1. Thus, there was a greater elevation in cyclic GMP in 1K1C rabbits, but this did not result in a corresponding greater depression in O2 consumption. This suggests that cyclic GMP plays a role in the control of myocardial metabolism, and that the sensitivity of myocardial O2 consumption to changes in cyclic GMP is reduced by renal hypertension-induced cardiac hypertrophy.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00803752
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  • 3
    Digitale Medien
    Digitale Medien
    The negative functional and metabolic effects of muscarinic stimulation are enchanced byβ-adrenergic activation in control and hypertrophic dog hearts in vivo (1997)
    Springer
    Basic research in cardiology 92 (1997), S. 391-401 
    Details
    ISSN: 1435-1803
    Schlagwort(e): Cardiac hypertrophy ; acetylcholine ; dog ; β-adrenergic stimulation ; myocardial O2 consumption ; regional myocardial function
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract The aim of the current study was to determine if the effects of muscarinic stimulation on left ventricular function and metabolism are greater during β-adrenergic activation, whether a cyclic GMP-mediated mechanism is responsible, and if this is altered by left ventricular hypertrophy (LVH) induced by aortic valve stenosis. Acetylcholine (Ach) (5 μg/kg/min) and/or isoproterenol (Iso) (0.1 μg/kg/min) was infused into a branch of the left anterior descending (LAD) artery in 8 control and 8 LVH open-chest anesthetized dogs. LVH increased heart weight, heart-to-body weight ratio and systolic left ventricular pressure. LVH reduced muscarinic receptor density (fmol/mg protein) (control: 149.2±18.6; LVH: 77.8±8.6), but not affinity. Alone, Ach had no effect on regional force, work or metabolism. Iso increased peak force (g) (control: baseline-7.4±0.4; Iso-12.4±2.2; LVH: baseline-6.7±0.8; Iso-16.3±2.7, regional work (g mm/min)) (control: baseline-1250±186; Iso-1813±409; LVH: baseline-927±235; Iso-1244±222), and O2 consumption (ml O2/min/100 g) (control: baseline-3.3±0.2; Iso-8.1±2.0; LVH: baseline-4.8±1.0; Iso-8.3±1.1). During Iso, Ach reduced segment shortening (control: Iso-14.5±1.2; Iso+Ach-10.5±1.8; LVH: Iso-10.4±1.5; Iso+Ach-7.6±1.3) and peak force (control: Iso+Ach-7.7±1.0; LVH: Iso+Ach-10.5±1.4). Ach also reduced work (control: Iso+Ach-875±217; LVH: Iso+Ach-776±180) and O2 consumption (control: Iso+Ach-3.4±0.7; LVH: Iso+Ach-3.6±0.6) in the presence of Iso. Cyclic GMP was higher in the LVH animals during all treatments and was elevated from baseline by Ach in both groups. Neither Iso nor Iso+Ach had a significant effect on cyclic GMP. Thus, the negative functional and metabolic effects of muscarinic stimulation are enhanced during β-adrenergic activation. This does not, however, appear to be dependent on a cyclic GMP-mediated mechanism. Despite reduced number of muscarinic receptors, this response was not altered by pressure-induced cardiac hypertrophy.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00796213
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