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  • 1
    Digitale Medien
    Digitale Medien
    Voltage sensitivity of NMDA-receptor mediated postsynaptic currents (1990)
    Springer
    Experimental brain research 81 (1990), S. 209-212 
    Details
    ISSN: 1432-1106
    Schlagwort(e): NMDA ; Excitatory postsynaptic current ; Voltage sensitivity ; Patch clamp ; Thin hippocampal slice ; Rat
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary Patch-clamp techniques were used to record pharmacologically-isolated N-methyl-D-aspartate-mediated excitatory postsynaptic currents (NMDA-EPSCs) from dentate granule cells in thin rat hippocampal slices. Membrane voltage modulated these EPSCs in two ways. Firstly, depolarization from resting potential enhanced EPSC amplitudes, as expected for a voltage-dependent block by Mg2+ of synaptically activated NMDA receptor channels. Secondly, depolarization markedly prolonged the time course of decay of NMDA-EPSCs in normal and low extracellular Mg2+. Both mechanisms were complementary in establishing a strong dependence between membrane potential and the amount of charge, namely Ca2+, transferred through synaptically activated NMDA receptor channels, that presumably underlies induction of long-term potentiation in the hippocampus.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00230117
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    A thin slice preparation for patch clamp recordings from neurones of the mammalian central nervous system (1989)
    Springer
    Pflügers Archiv 414 (1989), S. 600-612 
    Details
    ISSN: 1432-2013
    Schlagwort(e): Patch clamp ; Brain slice ; Central nervous system
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract (1) A preparation is described which allows patch clamp recordings to be made on mammalian central nervous system (CNS) neurones in situ. (2) A vibrating tissue slicer was used to cut thin slices in which individual neurones could be identified visually. Localized cleaning of cell somata with physiological saline freed the cell membrane, allowing the formation of a high resistance seal between the membrane and the patch pipette. (3) The various configurations of the patch clamp technique were used to demonstrate recording of membrane potential, whole cell currents and single channel currents from neurones and isolated patches. (4) The patch clamp technique was used to record from neurones filled with fluorescent dyes. Staining was achieved by filling cells during recording or by previous retrograde labelling. (5) Thin slice cleaning and patch clamp techniques were shown to be applicable to the spinal cord and almost any brain region and to various species. These techniques are also applicable to animals of a wide variety of postnatal ages, from newborn to adult.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00580998
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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