ISSN:
1432-2242
Keywords:
Brassica napus
;
Brassica hirta
;
Somatic hybridization
;
mtDNA
;
ctDNA
;
rDNA
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Somatic hybridization between Brassica napus and B. hirta (or Sinapis alba) is described. No cybrid plant with B. napus nucleus exhibiting cytoplasmic male sterility was recovered. Somatic hybrids were identified morphologically and, for some of them, by cytological observations. They were also characterised by Southern hybridization of nuclear rDNA. Chloroplast and mitochondrial DNA restriction analysis showed that 2 plants out of 14 have B. hirta ctDNA, one the B. napus mtDNA and the other a hybrid. Nine possess B. napus ctDNA with a hybrid mtDNA. For six of them, mtDNA patterns present novel bands, suggesting intergenomic recombination during fusion. These hybrids will be included in the breeding program.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00289119
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