ISSN:
1432-1424
Schlagwort(e):
Intracellular ions
;
X-ray microanalysis
;
Sodium
;
Chloride
;
Bromide
;
Frog skin epithelium
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Biologie
,
Chemie und Pharmazie
Notizen:
Abstract Intracellular ion concentrations were determined in split skins of Rana pipiens using the technique of electron microprobe analysis. Based on the 1 min Br uptake from the apical bath, two types of mitochondria-rich (MR) cells could be distinguished: active cells which rapidly exchanged their anions with the apical bath and inactive cells which did not. Br uptake and frequency of active MR cells were closely correlated with the skin conductance, g t. Replacing Cl in the apical bath with an impermeant anion significantly lowered g t and the Br uptake and Na concentration of active cells. Even larger reductions were observed after apical amiloride (0.1 mm). The inhibition of the Br uptake was reversible by voltage clamping (100 mV, inside positive). Cl removal and amiloride also led to some shrinkage of active cells. The results suggest that the active cell is responsible for a large part of g t. Inactive MR cells had much lower Br and Na concentrations which were not significantly affected by Cl removal, amiloride, or voltage clamping. Principal cells, which represent the main cell type of the epithelium, showed only a minimal Br uptake from the apical side which was not correlated with g t. Moreover, Cl removal had no effect on the Na, Br, and Cl concentrations of principal cells.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/BF00232645
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