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Pulse response in an immobilized-enzyme column: Theoretical method for predicting elution curves (1980)

Adachi, S. ; Hashimoto, K. ; Matsuno, R. ; [et al.]

New York, NY [u.a.] : Wiley-Blackwell

Biotechnology and Bioengineering 22 (1980), S. 779-797

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ISSN: 0006-3592

Keywords: Chemistry ; Biochemistry and Biotechnology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology

Notes: A theoretical method for predicting the elution profile of a pulse response in an immobilized-enzyme column is proposed. The method is based on a mass balance model, which is extensively used in gel chromatography. To test the method, a pulse of sucrose solution was applied to a column of spherical acrylamide gel in which was entrapped invertase from yeast, and it was eluted with 0.05M acetate buffer at pH 5.0. The elution curves of the substrate and the product were in fairly good agreement with the theoretically calculated ones. The method was extended to the system of reversible as well consecutive reactions.

Additional Material: 9 Ill.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1002/bit.260220405

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CRF-immunoreactive nerve fibers in the circumventricular organs of the monkey, Macaca fuscata (1983)

Kawata, M. ; Hashimoto, K. ; Takahara, J. ; [et al.]

Springer

Cell & tissue research 232 (1983), S. 679-683

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ISSN: 1432-0878

Keywords: CRF-immunoreactive nerve fibers ; Circumventricular organs ; Immunohistochemistry ; Monkey, Macaca fuscata

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Medicine

Notes: Summary The occurrence of CRF (corticotropin-releasing factor)-immunoreactive nerve fibers in the circumventricular organs of adult male monkeys, Macaca fuscata, was studied on serially sectioned brains, by means of the peroxidase-antiperoxidase technique in combination with a highly specific and sensitive CRF antiserum. CRF-containing nerve fibers were found in high concentrations in the infundibulum and, in addition, in small numbers in the posterior lobe, organum vasculosum laminae terminalis, subfornical organ, and area postrema; they were missing in the pineal body and the subcommissural organ. The CRF immunoreactive nerve fibers distributed in these organs were located in the proximity of the blood vessels.