ISSN:
0170-2041
Schlagwort(e):
Preparative-enzymatic synthesis
;
Cytidine 5′-triphosphate (CTP)
;
Cytidine 5′-monophosphosialate synthase
;
Cytidine 5′-monophosphosialate (CMP-Neu5Ac)
;
CTP regeneration, integrated
;
Chemistry
;
Organic Chemistry
Quelle:
Wiley InterScience Backfile Collection 1832-2000
Thema:
Chemie und Pharmazie
Notizen:
The preparative formation of cytidine 5′-triphosphate by an enzymatic process with adenylate kinase and pyruvate kinase is described. The enzymes may either be used free, in the “MEEC” technique, or immobilized on a synthetic copolymer of vinyl acetate and N,N′-divinylethyleneurea (“VA-Epoxy”). Cytidine 5′-monophosphosialate synthase (preferably isolated from calf brain) and inorganic pyrophosphatase were used to prepare the activated neuraminic acid cytidine 5′-monophosphosialate (CMP-Neu5Ac) from CTP and neuraminic acid in good yield. Finally, both processes could be integrated and led to an in situ approach which, owing to moderate yields, requires further development.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1002/jlac.1990199001199
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