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  • 1
    ISSN: 1432-2013
    Keywords: Key words Ruthenium red ; Smooth muscle ; Ca2+-dependent K+ channel ; Ca2+ channel ; Ryanodine ; Urinary bladder
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  Three major ionic currents, Ca2+-dependent K+ current (I K-Ca), delayed rectifier type K+ current (I kd) and Ca2+ current (I Ca), were activated by depolarization under whole-cell clamp in single smooth muscle cells isolated from guinea-pig urinary bladder. Externally applied ruthenium red (RuR) reduced the amplitude of I K-Ca and I Ca at 0 mV (IC50 values were 4.2 and 5.6 μM, respectively), but did not affect I Kd. Spontaneous transient outward currents (STOCs) and caffeine-induced outward currents (I caf) at –30 mV were reduced by external 10 μM RuR. When 10 μM RuR was added to the pipette solution, I K-Ca during depolarization, STOCs and I caf significantly decreased with time. RuR did not change the unitary current amplitude of the large-conductance Ca2+-dependent K+ (BK) channels, but reduced the open probability of the channel under excised patch-clamp recording mode. RuR reduced the channel activity more effectively from the cytosolic face than from the other. This inhibition decreased when the cytosolic Ca2+ concentration was increased. These results indicate that RuR blocks BK and Ca2+ channels in urinary bladder smooth muscle cells. The decrease in I K-Ca, STOCs and I caf by RuR is attributable to the direct inhibition of BK channel activity, probably in addition to the inhibition of Ca2+ release from storage sites. The direct inhibition of BK channel activity by RuR may be related to the interaction of RuR with the Ca2+-binding sites of the channel protein.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 0887-624X
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: In order to clarify the general kinetic behavior of emulsion polymerization initiated by oilsoluble initiators, the emulsion polymerization of styrene initiated by 2,2′-azoisobutyronitrile was as a typical example, investigated thoroughly. The variations of the polymerization rate and the number of polymer particles produced with changes in emulsifier (sodium lauryl sulfate), initiator, and monomer concentrations initially charged and the reaction temperature were determined. It is shown from these experimental results that the kinetic behavior of this emulsion polymerization system is quite similar to that of styrene emulsion polymerization initiated by the water-soluble initiator, potassium persulfate despite the difference in the principal loci of radical production in both systems.
    Additional Material: 14 Ill.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Hoboken, NJ : Wiley-Blackwell
    Journal of Biomedical Materials Research 14 (1980), S. 557-566 
    ISSN: 0021-9304
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine , Technology
    Notes: The mechanical properties of the hydrogel membrane of poly(methyl methacrylate-N-vinylpyrrolidone) were studied for the purpose of making soft contact lenses for extended wear. The dependence of tensile fracture energy, flexibility, and recovery from deformation on the water content and thickness of the hydrogel membrane was studied. Reducing the thickness of a lens was found to exert a more advantageous effect than increasing its water content on maximizing the tensile fracture energy under the condition of an adequate supply of oxygen to the cornea through the contact lens. As long as its water content is controlled between 63 and 78%, the contact lens made of poly(methyl methacrylate-N-vinylpyrrolidone) has the flexibility on the same order as that of conventional soft contact lenses of poly(2-hydroxyethyl methacrylate). The rate of recovery from deformation increases with water content, and the residual elongation is negligible in the range of water content over about 70%. It is concluded that practical requirements for use of these contact lenses, determined by these three mechanical properties, can be satisfied at the same time if the water content of the contact lens is adjusted at about 70%.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    New York : Wiley-Blackwell
    Journal of Polymer Science: Polymer Letters Edition 19 (1981), S. 143-147 
    ISSN: 0360-6384
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
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