ISSN:
1434-0879
Keywords:
Renal stone
;
Cell culture models
;
MDCK cell line
;
Calcium phosphate
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract Cell culture models of calcium phosphate renal stone formation were established using the MDCK cell line. Renal microliths were detected within pseudocysts in three-dimensional soft agar cultures, and were also observed in the basal region of cells lining the cell sheet, and immediately beneath domes or blisters in monolayers and collagen gel cultures. Light and scanning electron microscopy indicated that these microliths had a similar lamellated and spherical appearance to those in humans. These microliths were first detected microscopically after 21 days of culture, and were found to be composed of calcium phosphate by X-ray and microinfrared spectroscopic analyses. These culture models may provide a powerful new tool to study the pathogenesis of renal stone diseases and/or calcium phosphate stone formation in humans and animals.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00941907
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