ISSN:
1432-2013
Keywords:
Calmodulin
;
Myosin light chain kinase
;
Calmodulin antagonist
;
Smooth muscle skinned fibres
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract During smooth muscle activation the calcium calmodulin complex interacts with myosin light chain kinase (MLCK) whereby activating it. A synthetic peptide analogue (RS20) corresponding to the calmodulin recognition sequence of MLCK has been synthesized and previously found to inhibit the calmodulin stimulated light chain kinase activity. Here we studied the effect of this peptide on skinned fibers from guinea pig taenia coli. Maximal contractions induced by 30 μM Ca2+ at 0.1 μM calmodulin could be completely relaxed by the peptide at 1 μM. The inhibitory effect was accompanied by partial dephosphorylation only of the regulatory myosin light chain. Relaxation could be reversed by addition of calmodulin which also increased the extent of light chain phosphorylation.The calmodulin concentration required for reversing the inhibition depended on the concentration of the inhibitory peptide suggesting that the peptide competed with MLCK for the calmodulin binding site. As the calcium-calmodulin-peptide mixture constitutes a calmodulin buffer, our results suggest, that the peptide is a calmodulin antagonist unique in terms of its potency and that less than nanomolar concentrations of free calmodulin may be required for inducing smooth muscle contractions.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00584627
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