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  • Cell & Developmental Biology  (2)
  • Column liquid chromatography  (2)
  • Fe-Versorgung  (2)
  • 1
    ISSN: 1436-6215
    Keywords: Fe-Versorgung ; Proteinversorgung ; Fe-Protein-Wechselwirkung ; Fe-Konzentration in verschiedenen Organen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
    Description / Table of Contents: Summary In a 2×3factorial experiment with growing rats the diet varied for the iron content (5, 25, 625 ppm) and for the protein content (5, 25, 45 %). The diets were fed ad libitum for 35 days. At the end of the experiment the iron content of different organs was determined. Both experimental factors and their interactions influenced the iron concentration as well as fresh weight and dry matter dependent on organ. Especially the influence of deficient protein supply and of insufficient iron supply was very marked. Dependent on the iron supply there are different responses between storage and functional compounds of iron.
    Notes: Zusammenfassung In einem 2×3faktoriellen Versuch mit wachsenden Ratten variierte der Fe-Gehalt der Diät in drei Stufen (5, 25, 625 ppm) und der Proteingehalt ebenfalls in drei Stufen (5, 25, 45 %). Die Diäten wurden den Versuchstieren 35 Tage ad libitum verfüttert und nach Versuchsende der Fe-Gehalt in verschiedenen Organen bestimmt. Die beiden Versuchsfaktoren und deren Wechselwirkungen beeinflußten die Fe-Konzentration sowie Frischgewicht und Trockensubstanzgehalt je nach Organ. Besonders starken Einfluß übten sowohl eine mangelhafte Proteinzufuhr als auch eine nicht bedarfsgerechte Fe-Versorgung aus. In Abhängigkeit von der Fe-Versorgung zeigten sich zwischen Speicherverbindungen und funktionellen Verbindungen für Eisen unterschiedliche Reaktionen.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    European journal of nutrition 23 (1984), S. 20-30 
    ISSN: 1436-6215
    Keywords: Cu-Versorgung ; Fe-Versorgung ; Cu-Fe-Wechselwirkungen ; Cu-Fe-Gehalte in Organen und Geweben
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
    Description / Table of Contents: Summary The Fe and Cu contents in the femur and in the skeleton muscle were investigated in a trial series with factorial Fe and Cu supplies of growing rats (0, 25, 250, and 625 μg of Fe supply; 0, 10, 100, and 250 μg Cu supply per g of the diet). In addition, the reaction of the carcass to the different trace element supply was compared to the changes of the Fe and Cu contents in organs and tissues. In the femur, the Fe contents clearly increase in all grades of the Cu supply with increasing Fe supply. But because of the reduced live weight of the trial animals without Fe supply, this is valid only from 25 μg Fe/g diet on. For all Fe levels, the influence of the Cu supply on the concentration and the content of Cu results in a plateau in the range of 10–100 μg Cu/g diet; it is not reached with Cu deficiency and exceeded with an excessive Cu supply (250 μg/g). A clear increase of the Fe concentration in the muscle occurs only at a supply of 250 μg Fe/g diet. A further increase at 625 μg Fe/g only occurs at a Cu supply of 250 μg/g. Generally, the Fe concentration is reduced in the Cu deficiency. The Cu concentration in the muscle increases in all cases with the Cu supply, and the extent of this increase is strongly influenced by the Fe supply. The Fe contents in the carcass primarily depend on the Fe dosage. For the total contents of copper there are hints of a homeostatical regulation in the range of 10–100 μg Cu/g, but it seems to be disturbed if the Fe supply (0 or 25 μg/g) is insufficient. The comparison of the Fe and Cu contents in organs and tissues to those in the carcass shows that the reactions to the different supply levels, which are clearly different in the tissues, can be reproduced but insufficiently by the trace element analysis in the carcass.
    Notes: Zusammenfassung Im Rahmen einer Versuchsreihe mit faktorieller Fe- und Cu-Versorgung wachsender Ratten (0, 25, 250 und 625 μg Fe-Zulage; 0, 10, 100 und 250 μg Cu-Zulage je g Diät) wurden die Fe- und Cu-Gehalte im Femur und Skelettmuskel untersucht. Zusätzlich wurde die Reaktion des Ganzkörpers auf die unterschiedliche Spurenelementzufuhr mit der Veränderung des Fe- und Cu-Gehaltes in Organen und Geweben verglichen. Im Femur steigen die Gehalte an Eisen bei allen Stufen der Cu-Versorgung deutlich mit der Fe-Zufuhr an. Bedingt durch die verringerte Lebendmasse der Versuchstiere ohne Fe-Zulage gilt dieser Zusammenhang erst ab 25 μg Fe/g Diät. Der Einfluß der Cu-Versorgung auf die Konzentration und den Gehalt an Kupfer ergibt für alle Fe-Stufen ein Plateau im Bereich von 10–100 μg Cu/g Diät, das bei Cu-Mangel in der Regel unter-, bei exzessiver Cu-Zufuhr (250 μg/g) überschritten wird. Im Muskel erfolgt ein deutlicher Anstieg der Fe-Konzentration erst bei einer Zufuhr von 250 μg Fe/g Diät. Durch 625 μg Fe/g tritt nur bei einer Cu-Zulage von 250 μg/g eine weitere Erhöhung auf. Im Cu-Mangel ist die Fe-Konzentration in der Regel erniedrigt. Die Cu-Konzentration im Muskel steigt in allen Fällen mit der Cu-Zufuhr an, wobei das Ausmaß dieses Anstiegs stark durch die Fe-Versorgung beeinflußt wird. Die Fe-Gehalte im Ganzkörper sind in erster Linie von der Fe-Dosierung abhängig. Für die Gesamtgehalte an Kupfer ergeben sich im Bereich von 10–100 μg Cu/g Hinweise auf eine homöostatische Regulation, die aber bei unzureichender Fe-Zufuhr (0 bzw. 25 μg/g) offensichtlich gestört ist. Der Vergleich der Fe- und Cu-Gehalte in Organen und Geweben mit denen im Gesamtkörper zeigt, daß die in einzelnen Geweben deutlich unterschiedlichen Reaktionen auf die einzelnen Versorgungsstufen durch die Spurenelementbestimmung im Ganzkörper nur sehr begrenzt wiedergegeben werden.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Column-switching ; Parathion, and metabolites in serum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary A new high-performance liquid chromatographic method with column-switching has been developed for the simultaneous determination of parathion and its metabolites such asp-nitrophenol and paraoxon in serum. Serum samples were injected onto a precolumn packed with LiChroprep RP-8 after simple dilution with 20% phosphoric acid. Polar plasma components were washed with 0.02 M phosphate buffer (pH 3.0). After valve switching, the concentrated compounds were eluted in back-flush mode and separated on a Ultracarb ODS 30 column with a gradient of acetonitrile −0.01 M phosphate buffer (pH 3.0) as mobile phase. The method showed excellent precision, accuracy and speed with detection limit of 0.1 μg mL−1. Total analysis time per sample was 〈40 min and coefficients of variation for intra-and inter-assay were 〈4.5%.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Microbore columns ; Column-switching ; Myristicin ; Serum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary A microbore high-performance liquid chromatographic method with column-switching was developed for the analysis of myristicin from rat serum without prepurification. Deproteinization, fractionation, concentration and separation of analyte were carried out by appropriate switching of columns and using solvent mixtures. The method showed excellent precision, accuracy and speed with a detection limit of 10 ng mL−1 from 25 μL of serum. The total analysis time per sample was 25 min and the coefficients of variation for intra- and inter-assay were less than 1.8%.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1040-452X
    Keywords: Fluo-3 ; Intracellular Ca2+ transient ; QNB ; Population kinetics ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The acrosome reaction induced by the zona pellucida in mouse sperm has been shown to proceed in two stages experimentally distinguishable by the fluorescent probe chlortetracycline. Entry into the first stage of sperm bound to isolated, structurally intact zonae pellucidae is blocked by the compound 3-quinuclidinyl benzilate. In this study, we show, utilizing the fluorescent Ca2+ indicator fluo-3, that the first stage of the zona-induced acrosome reaction is characterized by an increase in intracellular Ca2+, followed by a decrease as the acrosome reaction proceeds. This calcium transient is completely suppressed by 3-quinuclidinyl benzilate. We conclude that the Ca2+ transient is induced by the zona pellucida and is required for the zona-induced acrosome reaction. Blockage of this sperm intracellular Ca2+ transient provides a mechanism for the inhibitory action of 3-quinuclidinyl benzilate on the zona-induced acrosome reaction in mouse sperm. © 1992 Wiley-Liss, Inc.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    Journal of Electron Microscopy Technique 7 (1987), S. 161-166 
    ISSN: 0741-0581
    Keywords: Selective plasma etch ; Ceramic composites ; Transmission electron microscopy ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: Transmission electron microscopy has been used to isolate and examine the intergranular glass phase in hot-pressed silicon nitride/silicon carbide composites. Previously there have been difficulties in locating a suitable region for studies of this nature because the interfering nitride and carbide grains inhibit isolation of the glass for examination. Radiofrequency plasma etching of thinned sections of 6 wt% Y2O3, 2 wt% A12O3 in Si3N4 containing 30 vol% of SiC proved to be fruitful in isolating the glass phase. A mixture of CF4 and O2 quantitatively remove the acicular nitride phase without any evidence of attack on either the glass or carbide. Composites containing ceria and magnesia as substitutes for yttria behave similarly. This indicates that glasses containing minor to major concentrations of elements forming stable fluorides inhibit the attack of fluoride ions on silica glasses containing these elements.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
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