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Digitale Medien

Biological oxidation of nitric oxide in a humisol (1997)

Dunfield, P. F. ; Knowles, Roger

Springer

Biology and fertility of soils 24 (1997), S. 294-300

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ISSN: 1432-0789

Schlagwort(e): Key words Humisol ; Nitrate ; Nitric oxide ; Nitrite ; Nitrogen dioxide ; Acetylene

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie , Geologie und Paläontologie , Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft

Notizen: Abstract Consumption of nitric oxide (NO) in a humisol was studied at 0.1–2 ppmv NO, a range representative of NO concentrations in ammonium-fertilized soil. Denitrification was not a major sink for NO. The principal NO-consuming reaction was a biological oxidation, leading ultimately to nitrate (NO3 –). Nitrogen dioxide (NO2) and nitrite (NO2 –) may have been intermediates in this pathway. An abiological reaction accounted for about 25% of the NO-consuming activity in soil at 90% H2O (d.w.) and 25oC, but contributed relatively more to total NO consumption at higher temperatures. Biological NO-consuming activity was highest at 25oC, while the abiological activity increased exponentially with temperature. The product of the abiological reaction was neither NO3 –, NO2 –, nor nitrous oxide (N2O), and the reaction did not require O2.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/s003740050246

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Digitale Medien

Organization of actin and microtubules during process formation in tau-expressing sf9 cells (1994)

Knowles, Roger ; Leclerc, Nicole ; Kosik, Kenneth S.

New York, NY : Wiley-Blackwell

Cell Motility and the Cytoskeleton 28 (1994), S. 256-264

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ISSN: 0886-1544

Schlagwort(e): taxol ; cytochalasin ; polarity ; microtubule-associated proteins ; lammelopodia ; Life and Medical Sciences ; Cell & Developmental Biology

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Biologie , Medizin

Notizen: Insect Sf9 cells usually elaborate a highly characteristic single process when infected with a baculovirus encoding recombinant human tau. The processes are unbranched, of uniform caliber, and contain bundles of microtubules. Because taxol treatment alone does not induce process outgrowth in these cells, it is believed that tau confers properties on microtubules that permits the conversion of microtubule assembly into the formation of processes. Here we have analyzed the reorganization of both actin filaments and microtubules during process initiation. A zone of organelle exclusion representing the focal reorganization of actin at one pole of the cell anticipated process emergence. A relationship between actin organization and process emergence was also suggested by a shift from single to multiple process formation after treatment with cytochalasin D. The rate of process elongation doubled after cytochalasin treatment of tau-expressing cells. The increase in rate was due to the inhibition of the growth arrest phases which occur in the absence of cytochalasin. In contrast, Sf9 cells treated with cytochalasin after more than 20 h of tau expression were relatively resistant to the drug's effects. We conclude that actin and microtubules are specifically reorganized during tau-induced process outgrowth and that a dynamic relationship between actin filaments and microtubules effects process formation. © 1994 Wiley-Liss, Inc.

Zusätzliches Material: 5 Ill.