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  • 1
    Electronic Resource
    Electronic Resource
    Growth characteristics of human pituitary adenomas in tissue and cell cultures (1980)
    Springer
    Acta neuropathologica 49 (1980), S. 225-230 
    Details
    ISSN: 1432-0533
    Keywords: Human pituitary adenomas ; Tissue culture ; Cell culture ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Pituitary adenomas of 120 patients were investigated in tissue and in cell cultures. Under different conditions of culture, the biopsies revealed different rates of growth. In tissue cultures 66% of the samples could be propagated while tumour specimens explanted in cell suspensions proliferated in 80% of the cases. Attempts to establish subcultures were also more successful when specimens were put into cell cultures primarily. Further differences of growth characteristics were observed in connection with the histological types of the parent tissues. According to the old terminology cells of mixed type adenomas had the longest life-span both in tissue and in cell cultures. Considering the proliferative activity, this type of tumour could be most frequently subcultured. Concerning the relationship of different cell components of the biopsies it was proven that survival and proliferation of adenoma cells may occur without fibroblasts. The appearance of fibroblast-like cells in older cultures is a morphological manifestation of the senescence of the specific cell types. Qualitative features of cultured adenoma cells showed that on the basis of cytomorphological properties “chromophobe” samples could be separated from the other types. Cells of acidophil and mixed type growths had the capacity of developing in vitro various migratory shapes, while “chromophobe” cells did not possess this ability. The histological diagnoses made independently from cultures confirmed the tissue culture findings. Differences of in vitro characteristics correlated also with the ultrastructural features of the cultured adenoma cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00707110
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Light- and electron-microscopic features of an endocrinologically active pituitary adenoma cultured in vitro (1982)
    Springer
    Acta neuropathologica 56 (1982), S. 245-249 
    Details
    ISSN: 1432-0533
    Keywords: Pituitary adenoma ; Cell culture ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A pituitary adenoma from an acromegalic female patient has been studied in cell cultures. Two populations of parenchymal cells, i.e., elongated shapes and big pale forms with irregular outlines, were distinguished light-microscopically. Ultrastructurally, three types of cells were found. Two of them contained secretory granules in varying numbers and sizes. Based on the proportion of the various cellular elements these cells were considered as counterparts of the elongated shapes being responsible for the GH secretion at different rates. The third cell type with very few secretory granules was identified with the big pale forms. Presumably, they could be exhausted GH-secreting cells. Besides fine structural characterisation, cultured cells of endocrinologically active human pituitary adenomas offer suitable models for studying some phases of exocytosis and membrane retrieval.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00691254
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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