Library

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Electronic Resource
    Electronic Resource
    Cloning of caf1 +, caf2  + and caf4  + from Schizosaccharomyces pombe : their involvement in multidrug resistance, UV and pH sensitivity (1998)
    Springer
    Molecular genetics and genomics 260 (1998), S. 434-443 
    Details
    ISSN: 1617-4623
    Keywords: Key words Schizosaccharomyces pombe ; Caffeine resistance ; Brefeldin A resistance ; Multidrug resistance ; Cell cycle
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We previously identified four nuclear genes (caf1 + to caf4 +) in Schizosaccharomyces pombe, mutations in which can confer caffeine resistance. Here we report the cloning and sequencing of caf1 +, caf2 + and caf4 +. All three genes are allelic to genes (hba1 + , crm1 + and trr1 + , respectively) involved in multidrug resistance mechanisms or in stress response systems. In agreement with this the caffeine-resistant mutants caf1(hba1)-21, caf2(crm1)-3 and caf4(trr1)-83 are also resistant to brefeldin. Disruption of caf1(hba1) + and caf4(trr1) + makes cells sensitive to high pH. The overlapping ranges of pleiotropic effects and the genetic interaction detected between caf1(hba1) + and caf2(crm1) + suggest that the three genes function in interlinked systems.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004380050914
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Molecular cloning and sequence analysis of mutant alleles of the fission yeast cdc2 protein kinase gene: Implications for cdc2 + protein structure and function (1989)
    Springer
    Molecular genetics and genomics 218 (1989), S. 41-49 
    Details
    ISSN: 1617-4623
    Keywords: Protein kinases ; cdc2 ; Schizosaccharomyces pombe ; Cell cycle ; Mitotic control
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The cdc2 + gene function plays a central role in the control of the mitotic cell cycle of the fission yeast Schizosaccharomyces pombe. Recessive temperature-sensitive mutations in the cdc2 gene cause cell cycle arrest when shifted to the restrictive temperature, while a second class of mutations within the cdc2 gene causes a premature advancement into mitosis. Previously the cdc2 + gene has been cloned and has been shown to encode a 34 kDa phosphoprotein with in vitro protein kinase activity. Here we describe the cloning of 11 mutant alleles of the cdc2 gene using two simple methods, one of which is presented here for the first time. We have sequenced these alleles and find a variety of single amino acid substitutions mapping throughtout the cdc2 protein. Analysis of these mutations has identified a number of regions within the cdc2 protein that are important for cdc2 + activity and regulation. These include regions which may be involved in the interaction of the cdc2 + gene product with the proteins encoded by the wee1 +, cdc13 + and suc1 + genes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00330563
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Molecular analysis of hus1 +, a fission yeast gene required for S-M and DNA damage checkpoints (1997)
    Springer
    Molecular genetics and genomics 254 (1997), S. 389-399 
    Details
    ISSN: 1617-4623
    Keywords: Key words Fission yeast ; Checkpoint ; Cell cycle ; Hus1
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The structure of hus1 +, a Schizosaccharomyces pombe gene required for S-M and DNA damage checkpoints, has been determined. Expression of hus1 + requires splicing of five exons, including a microexon that is only 13 nucleotides long. hus1 + is predicted to encode a 33 kDa protein with no similarity to sequences in any database, including the entire S. cerevisiae genome. Yeast strains disrupted for the hus1 + gene are viable but checkpoint-defective. Polyclonal antibodies were raised against bacterially expressed Hus1 protein, and used to study Hus1 regulation. Hus1 protein levels are not affected by S-phase arrest, and are not altered by mutations in other checkpoint genes, suggesting that Hus1 is not regulated at the transcriptional or translational levels.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/PL00008606
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...