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  • 1
    ISSN: 1573-7276
    Keywords: CD44 ; cell adhesion ; hyaluronic acid ; invasion ; mesothelial cells ; metastasis ; ovarian tumour cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Our previous studies have suggested that the interaction between hyaluronic acid (HA) on peritoneal mesothelial cells and the membrane adhesion molecule, CD44, on ovarian tumour cells could be important in ovarian cancer metastasis. In order to study this further, adhesion of six ovarian tumour lines to HA coated on to a plastic surface was investigated. Four lines bound to the HA coat and two lines did not. The adhesive lines were those that expressed high amounts of CD44, but the degree of adhesion was not closely correlated with CD44 expression. The results suggested that different tumour lines had different affinities for HA. Treatment of the HA coat with hyaluronidase substantially reduced adhesion. Adhesion was also partially reduced if the tumour cells were preincubated with either soluble HA, or anti-CD44 antibodies directed against the HA binding region. An antibody against a non-HA binding region only slightly blocked adhesion at high antibody concentrations. Only the CD44H isoform was detected by immunoprecipitation on the tumour cells. These results suggest that ovarian tumour cells can attach to immobilised HA via CD44H on the cell membrane.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The response of a packed bed to a sine wave of temperature in a stream of fluid through it will depend upon the amount of dispersion in the resistance to transfer between fluid and solid, and the thermal properties of the soild. A method is presented that allows the effects of these three phenomena on the amplitude and phase angle to be unravelled and hence all their magnitudes to be computed simultaneously. It thus presents a way of determining these three quantities in situations where they were previously obtainable either with great uncertainty or not at all. The method requires measurements of the relative amplitudes and phase angles at three frequencies, and (preferably) at ω → 0 as well, all to be carried out with high accuracy.
    Additional Material: 1 Tab.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The method of computing the numerical values is given. It is illustrated with a simulated example to show the high accuracy that is possible, the unknowns being found to four significant figures. The only quantities required are ones that can be measured accurately: the radius of the spherical packing, its volumetric specific heat, and values of the amplitude attenuation and phase angle lag at a minimum of four finite values of the frequency.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
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