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  • 1
    Electronic Resource
    Electronic Resource
    X-ray microanalysis of rat mast cells stimulated with compound 48/80 in combination with quick-freezing method (1994)
    Springer
    Virchows Archiv 425 (1994), S. 435-438 
    Details
    ISSN: 1432-2307
    Keywords: Mast cell ; Quick-freezing ; Compound 48/80 ; X-ray microanalysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract X-ray microanalysis was performed on rat mast cells prepared by quick-freezing, cryosectioning and freeze-drying (QF-FD) method, or quick-freezing and freeze-substitution (QF-FS) method. Peritoneal cells including mast cells were stimulated with compound 48/80 for 0, 10 or 30 s at 17° C, and the mast cells stimulated for 30 s started exocytosis. In X-ray spectra of the QF-FD specimen, mast cells stimulated for 10 s increased their levels of phosphorus, sodium and chlorine in the intergranular cytoplasm prior to exocytosis, and kept this increase until 30 s after stimulation. In the QF-FS specimen, where soluble elements were removed, peaks of phosphorus, sulphur and potassium could be detected as elements in X-ray spectra. Phosphorus increased and potassium decreased in intergranular cytoplasm of mast cells stimulated for 10 s, and these changes became more obvious after 30 s. However, supplemental increase of other cations such as sodium could not be detected in the QF-FS specimens.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00189582
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  • 2
    Electronic Resource
    Electronic Resource
    Freeze-fracture immunocytochemistry for intracellular localization of serotonin in mast cells stimulated with compound 48/80 (1995)
    Springer
    Virchows Archiv 426 (1995), S. 267-270 
    Details
    ISSN: 1432-2307
    Keywords: Mast cell ; Compound 48/80 ; Exocytosis ; Freeze-fracture immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Changes of intracellular localization of serotonin in rat mast cells were examined by freeze-fracture immunocytochemistry, to prevent the translocation of the serotonin antigen. Rat peritoneal cells including mast cells were stimulated in vitro with compound 48/80, at 17° C for 0, 30 or 60 s for exocytosis to occur. The mast cells were fixed, quickly frozen and freeze-fractured to expose the antigen on the fractured surface. They were immunostained with serotonin antibody, and the immunoreactions on the fractured surface were examined on ultrathin sections by electron microscopy. Unstimulated mast cells exhibited serotonin localization mostly in each intragranular matrix. In contrast, mast cells stimulated for 30 s exhibited increased serotonin in their intergranular cytoplasm. Mast cells showed more distinct immunoreactions in the cytoplasm where degranulation would be promoted after 60 s. It is suggested that intracellular release of serotonin occurred in the stimulated mast cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00191364
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  • 3
    Electronic Resource
    Electronic Resource
    Ultrastructural study of mast cells stimulated with compound 48/80 as revealed by quick-freezing method (1994)
    Springer
    Virchows Archiv 424 (1994), S. 287-294 
    Details
    ISSN: 1432-2307
    Keywords: Mast cell ; Compound 48/80 ; Ultrastructure ; Quick-freezing
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The ultrastructure of mast cells stimulated with compound 48/80 was examined by quick-freezing and deep-etching (QF-DE) or freeze-substitution (QF-FS) methods. Peritoneal cells including mast cells of adult male rats were stimulated in vitro with compound 48/80 at 17° C for 0, 10, 30, 60 or 180 s. The QF-DE replicas revealed that the mast cells stimulated with compound 48/80 for 30 s decreased filamentous actin around secretory granules. In the QF-FS specimens, perigranular membranes in mast cells stimulated for 60 s formed pentalaminar structures between adjacent granules in their cytoplasm prior to degranulation. These findings suggest that preparatory states for degranulation occur in the whole cytoplasm of stimulated mast cells at early stages. Moreover, both QF-FS specimens and QF-DE replicas revealed a compact morphological appearance of discharged granules in the extra-cellular space, indicating the existence of considerable content within the granules. Skeletal structures in the granules were also demonstrated on QF-DE replicas prepared after extracting soluble elements from the cytoplasm. It is suggested that the granular contents associated with the skeletal structures are gradually detached from the discharged granules to ensure local concentration in the tissues.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00194613
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  • 4
    Electronic Resource
    Electronic Resource
    Separation and determination of purpurea glycosides in Digitalis purpurea leaves by micro-HPLC (1987)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 10 (1987), S. 137-140 
    Details
    ISSN: 0935-6304
    Keywords: Liquid chromatography, Micro-HPLC ; Purpurea glycosides ; Digitalis purpurea ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: A micro high-performance liquid chromatographic (micro-HPLC) method was developed for the determination of purpurea glycoside A, purpurea glycoside B, and glucogitaloxin in Digitalis purpurea leaves. The extract of dry leaf powder was applied to a Sep-Pak silica cartridge followed by preparative thin-layer chromatography prior to micro-HPLC analysis. The analysis was performed on an ODS micro column, using acetonitrile-methanol-water (21:20:45) and ultraviolet detection (220 nm). The amounts of purpurea glycoside A, purpurea glycoside B, and glucogitaloxin per 100 mg of dry leaf powder were estimated to be 52.6, 50.7, and 108.6 μg, respectively.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240100306
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  • 5
    Electronic Resource
    Electronic Resource
    On the Structure of Graphite Fluoride (1987)
    Weinheim : Wiley-Blackwell
    Zeitschrift für anorganische Chemie 544 (1987), S. 7-20 
    Details
    ISSN: 0044-2313
    Keywords: Chemistry ; Inorganic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Zur Struktur von GraphitfluoridDie Struktur von Graphitfluorid, (C2F)n, wurde mittels Röntgenanalyse, 19F-Festkörper-NMR und Elektronenmikroskopie an gut charakterisierten und kristallisierten Proben von natürlichem Graphit und HOPG (highly oriented pyrolytic graphite) untersucht. Aufgrund dieser Ergebnisse und den in vorhergehenden Arbeiten erhaltenen strukturellen Eigenschaften hat (C2F)n eine Schichtstruktur der 2. Stufe, die zum hexagonalen System mit der Symmetrie C3h gehört. Detallierte Diskussionen zur Symmetrie sowohl für (CF)n wie für (C2F)n haben zur möglichen Stapelsequenz geführt, die jede Einheitszelle des Graphitfluorids erfordern sollte. Die Idealstruktur ist ein hexagonales Kristallgitter mit a = b 2,5 Å; c = 16,2 Å und einer plausiblen Stapelsequenz von AB/B′A′/ mit der Identitätsperiode Ic = 8,09 Å. Die Schichtstruktur von (CF)n ist von 1. Stufe mit der Stapelsequenz A/A′/.
    Notes: The structure of graphite fluoride, (C2F)n has been investigated by X-ray analyses, solid state 19F-n.m.r., and electron microscopy for well characterized and crystallized samples obtained from natural graphite or HOPG (highly oriented pyrolytic graphite). On the basis of the present results and structural properties derived from previous works, (C2F)n has a layered structure of stage-2 which belongs hexagonal to the system with C3h symmetry. Detailed discussions on the symmetry both for (CF)n and (C2F)n have led to possible stacking sequences each unit cell of graphite fluoride should require. The ideal structure of (C2F)n is a hexagonal crystal lattice with a = b = 2.5 Å; c = 16.2 Å, and a plausible stacking sequence of AB/B′A′/ with Ic (identity period) = 8.09 Å. The layered structure of (CF)n is of stage-1 with A/A′/ stacking sequence.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/zaac.19875440102
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