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  • 1
    Electronic Resource
    Electronic Resource
    Identification of the molecular species of lysophosphatidic acid produced when platelets are stimulated by thrombin
    Amsterdam : Elsevier
    Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism 1001 (1989), S. 282-285 
    Details
    ISSN: 0005-2760
    Keywords: (Human) ; Lysophosphatidic acid ; Phospholipase A"2 ; Platelet ; Thrombin
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Medicine , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0005-2760(89)90112-4
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  • 2
    Electronic Resource
    Electronic Resource
    Ultrastructure of the pars intermedia of the adult sheep hypophysis (1981)
    Springer
    Cell & tissue research 217 (1981), S. 211-223 
    Details
    ISSN: 1432-0878
    Keywords: Pituitary gland ; Pars intermedia ; Electron microscopy ; Sheep
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Light microscopy of coronal sections of the sheep pars intermedia revealed a compact, incompletely lobulated V-shaped region about 15–20 cells thick, situated between the pars distalis and the pars nervosa. A prominent hypophysial cleft and follicles containing a colloid-like substance were seen. Using electron microscopy, five cell types could be distinguished: pars intermedia glandular cells, pars distalis-like glandular cells, interstitial cells, follicular cells and cleft lining cells. The polyhedral to pear-shaped pars intermedia glandular cells predominated. They contained dense-cored, membrane-bound granules near the Golgi complex, and larger, irregular vesicles with finely granular contents of varying electron density throughout the remaining cytoplasm; exocytotic release of granules was occasionally observed. Smaller numbers of cells resembling those seen in the pars distalis were scattered throughout the pars intermedia. Interstitial cells usually possessed elongated cytoplasmic processes which extended between the glandular cells, and were characterized by deeply indented nuclei, elaborate junctional complexes and an absence of cytoplasmic granules. Cells lining the follicles resembled the interstitial cells. The major cells bordering the hypophysial cleft were triangular in section and bore irregular microvilli on their free surface. The pars intermedia appeared to be less vascular than the remainder of the hypophysis and only occasional fenestrated capillaries were seen. Nerve profiles were rare.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00233839
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  • 3
    Electronic Resource
    Electronic Resource
    Pilot scale affinity chromatography: Purification of β-galactosidase (1974)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 16 (1974), S. 1103-1112 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: β-Galactosidase has been purified from an ammonium sulfate precipitate of E. coli strain ML308 by biospecific adsorption on a column of agarose gel substituted with p-aminophenyl-β-D-thiogalactopyranoside. The system described using a 1.8 liter column has a useful processing capacity of 3.8 × 106 units of β-galactosidase per 2 hr cycle. This corresponds to about 5 g of pure enzyme. An electromechanical timing device operates a set of six solenoid valves and carries out a preset program consisting of sample application, washing, and elation operations.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260160810
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  • 4
    Electronic Resource
    Electronic Resource
    The properties of magnetic supports in relation to immobilized enzyme reactors (1973)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 15 (1973), S. 603-606 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260150318
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  • 5
    Electronic Resource
    Electronic Resource
    Stability of alginate-immobilized algal cells (1986)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 28 (1986), S. 210-216 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Investigations were carried out using immobilized Chlorella cells to determine the diameter, compressibility, tolerance to phosphate chelation, and ability to retain algal cells during incubation of various alginate beads. These physical bead characteristics were found to be affected by a variety of interactive factors, including multivalent cation type (hardening agent) and cell, cation, and alginate concentration, the latter exhibiting a predominant influence. The susceptibility of alginate beads to phosphate chelation was found to involve a complex interaction of cation type, concentration, and pH of phosphate solution. A scale of response ranging from gel swelling to gel shrinking was observed for a range of conditions. However, stable calcium alginate beads were maintained in incubation media with a pH of 5.5 and a phosphate concentration of 5μM. A preliminary investigation into cell leakage from the beads illustrated the importance of maintaining a stable gel structure and limiting cell growth to reduce leakage.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260280210
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  • 6
    Electronic Resource
    Electronic Resource
    Measurement of equilin and estrone in human plasma by capillary gas chromatography-negative ion chemical ionization mass spectrometry (1987)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 10 (1987), S. 6-11 
    Details
    ISSN: 0935-6304
    Keywords: Capillary gas chromatography ; Negative ion mass spectrometry ; Plasma ; Estrone ; equilin ; Conjugated estrogens ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: A procedure is described for the analysis of the estrogens equilin and estrone in human plasma following oral administration of conjugated estrogen preparations. After enzymatic hydrolysis of the sulfate conjugates, plasma proteins are precipitated with methanol and the estrogens extracted into ethyl acetate. Derivatization with the reagent flophemesylamine converts equilin and estrone into volatile pentafluorophenyldimethylsilyl ethers ideally suited to capillary gas chromatography-negative ion chemical ionization mass spectrometry. Using a 15 meter dimethyl silicone bonded phase fused silica capillary column separation of the estrone and equilin derivatives is achieved within 9 minutes. Selected ion monitoring of the intense negative molecular ions enables levels of 1 ng.ml-1 to be measured with coefficients of variation of 9.3 % and 14.2 % for estrone and equilin respectively. Plasma levels of the compounds are reported in two male volunteers up to 24 hours after dosing with 5 milligrams of Premarin™. (™ Ayerst Laboratories Inc., New York, USA.).
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240100102
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  • 7
    Electronic Resource
    Electronic Resource
    Ultrastructure of the pars intermedia of the developing sheep hypophysis (1982)
    Springer
    Cell & tissue research 224 (1982), S. 369-381 
    Details
    ISSN: 1432-0878
    Keywords: Pituitary gland ; Pars intermedia ; Electron microscopy ; Fetal sheep ; Lamb
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Using light and electron microscopy, the morphogenesis of the pars intermedia of the sheep pituitary gland was examined in developing lambs between 26 days of gestation and the newborn stage. Following the establishment of contact between the anterior and posterior lobe primordia seen at 26 days, the connection with the pharyngeal roof disappeared by 31 days. The lumen of Rathke's pouch, which was a prominent cavity at the earlier stages, became inconspicuous by 40 days but progressively increased in size during gestation and, in some newborn animals, contained colloid material. At 40 days, the pars intermedia consisted of a uniform population of undifferentiated cells. Cells with cytoplasmic granules were first identified at 50 days. The cytological appearance of granular cells at 70 days indicated increased synthetic activity and by 80 days they closely resembled adult glandular cells. At 100 days, membrane activity suggestive of exocytosis was first observed in granular cells; fenestrated capillaries were present, and early follicle formation between adjacent non-granular cells was seen. This apparent exocytotic release of granules was observed much more frequently between 100 days of gestation and the newborn stage than in adult pars intermedia cells. These findings indicate that glandular cells of the developing pars intermedia are actively engaged in synthesis, storage and secretion from an early stage.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00216880
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  • 8
    Electronic Resource
    Electronic Resource
    A method for the estimation of acetanilide, paracetamol and phenacetin in plasma and urine using mass fragmentographyAbbreviations: BSA = N, O-bis-(trimethylsilyl)acetamide: Regisil = bis-(trimethylsilyl)trifluoroacetamide; TRI-SIL Z = trimethylchlorosilane + BSA + trimethylsilylimidazole. (1976)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 3 (1976), S. 60-63 
    Details
    ISSN: 1052-9306
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Phenacetin, paracetamol and acetanilide can be determined in a plasma or urine sample by the use of deuterium labelled analogues. These are produced by reaction of hexadeuterioacetic anhydride with the appropriate aromatic amine. The -NHCOCD3 group is stable to hydrogen exchange below pH 8. The internal standard is added to the plasma or urine after enzymatic hydrolysis of the paracetamol conjugates and an ethyl acetate extract at pH 5 is evaporated under nitrogen and the residue derivatized with N, O-bis-(trimethylsilyl)-acetamide. An aliquot of this solution is injected into a g.c.m.s. system, and one ion characteristic of the material under study and the ion from the deuterium analogue (3 mass units greater) are monitored using a voltage switching technique. In the case of phenacetin, for example, ions at 251 and 254 are monitored. Calibration curves relating different weight ratios of the hydrogen and deuterium compounds to their respective signals from the gas chromatograph mass spectrometer are used to calculate the amount of a compound in a particular sample. These methods have been developed to study the oxidation of acetanilide to paracetamol and the de-ethylation of phenacetin to paracetamol. Preliminary results from experiments with phenacetin will be discussed.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bms.1200030204
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  • 9
    Electronic Resource
    Electronic Resource
    The identification of 6-methoxy 8-aminoquionoline as a metabolite of primaquine in man (1975)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 2 (1975), S. 304-306 
    Details
    ISSN: 1052-9306
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The identification of 6-methoxy 8-aminoquinoline as a metabolite of Primaquine, an important antimalarial drug, is described. The metabolite is present in urine, plasma and erythrocytes following drug ingestion. It was identified by mass fragmentography of its 1H and 2H acetate and the acetate produced from authentic material. No evidence of further metabolites was obtained.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bms.1200020605
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  • 10
    Electronic Resource
    Electronic Resource
    Single and multiple ion recording techniques for the analysis of diphenylhydantoin and its major metabolite in plasmaAbbreviations: DPH = diphenylhydantoin; HPPH = 5-(p-hydroxyphenyl)-5-phenylhydantoin; TMAH = trimethylanilinium hydroxide; BSA = N, O-bis-(trimethylsilyl)acetamide. (1977)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 4 (1977), S. 36-41 
    Details
    ISSN: 1052-9306
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: A method has been developed for single ion monitoring of diphenylhydantoin and its major metabolite 5-(p-hydroxyphenyl)-5-phenylhydantoin in plasma. A plasma extract is reacted with N, O-bis(trimethylsilyl)acetamide and single ion recording is carried out using a gas chromatograph mass spectrometer system. The mass value selected, m/e 254, is common to the TMS ethers of diphenylhydantoin and its principal metabolite 5-(p-hydroxyphenyl)-5-phenylhydantoin. The results indicate that one cause of an adverse reaction to diphenylhydantoin could be a reduced ability to hydroxylate the drug. Quantitative methods for the analysis of the drug and its major metabolite have also been developed. Diphenylhydantoin and 5-(p-hydroxyphenyl)-5-phenylhydantoin can be analysed in plasma after addition of deuterium labelled internal standards and conversion to volatile derivatives for mass fragmentographic analysis. Diphenylhydantoin and its internal standard are analysed as the N, N-dimethyl derivative, and the hydroxylated metabolite and its internal standard are converted to a pertrimetylsilyl compound by reaction with N, O-bis-(trimethylsilyl)acetamide.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bms.1200040104
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