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  • 1
    Electronic Resource
    Electronic Resource
    Induction of alkaloid diversity in hybrid plant cell cultures (1999)
    Springer
    Plant cell reports 18 (1999), S. 911-918 
    Details
    ISSN: 1432-203X
    Keywords: Key words Rauwolfia serpentina Benth. ex Kurz ; Rhazya stricta Decaisne ; Hybrid in vitro cultures ; Indole alkaloid induction ; Methyl jasmonate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The treatment of Rauwolfia serpentina x Rhazya stricta somatic hybrid cell suspension culture with 100 μM of methyl jasmonate led to a general increase in indole alkaloid content and to qualitative changes in the alkaloid pattern. The content of Six alkaloids were investigated with respect to their content in both the cell biomass and nutrition medium. Intracellular 17-O-acetyl-norajmaline content on the 5th day after treatment had increased about 40-fold compared with the control culture. The respective concentrations of the other alkaloids increased by a factor of two to five. In total 26 indole alkaloids were identified in extracts of the methyl jasmonate-treated culture by TLC, UV, MS and NMR data and comparison with reference alkaloids. The identification of macrophylline, yohimbine oxindole and yohimbine pseudoindoxyl has not been reported before in Rauwolfia serpentina or Rhazya stricta plants nor in cell cultures derived from these plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050683
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  • 2
    Electronic Resource
    Electronic Resource
    Exakte Gegenstromverteilung mit einfachsten Mitteln (1959)
    Weinheim : Wiley-Blackwell
    Chemie Ingenieur Technik - CIT 31 (1959), S. 351-352 
    Details
    ISSN: 0009-286X
    Keywords: Chemistry ; Industrial Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Es wird eine Apparatur beschrieben, die sich unter geringstem Material- und Zeitaufwand aus Reagensgläsern und Blech herstellen läßt, und bei der die Gleichgewichtseinstellung durch Einleiten von Gas relativ schnell erzielt wird. Der durch Kapillar-Überführung erzielte Genauigkeitsgrad wird an Hand einer Standard-Verteilung gezeigt.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cite.330310509
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  • 3
    Electronic Resource
    Electronic Resource
    A selected ion monitoring assay for tributyltin and its degradation productsThis work was presented, in part, at the 34th Annual Conference of the American Society for Mass Spectrometry, Cincinnati, Ohio, 1986. (1988)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 15 (1988), S. 565-569 
    Details
    ISSN: 0887-6134
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Tributyltin (TBT) is a biocide which has been shown to enter the aquatic environment by release from antifouling paints. TBT is acutely toxic to some marine organisms at concentrations near 1 μg I-1 and physiological changes may occur at low nanogram per liter concentrations. Gas chromatography/mass spectrometry (GC/MS) (methane chemical ionization) has been used for identification (full scanning) and quantification (selected ion monitoring) of TBT, dibutyltin (DBT) and monobutyltin (MBT). The butyltins were extracted from environmental water samples with hexane/0.2% tropolone and derivatized with hexyl magnesium bromide to form hexylbutyltins. Selected ion monitoring was at m/z 319 (TBT) and m/z 347 (DBT, MBT and tripentyltin, the internal standard). Calibration curves prepared in natural water were linear and detection limits were 〈2 ng I-1, GC/MS and GC with flame photometric detection were compared as quantification methods for environmental samples and were shown to give similar results at the low nanogram per liter levels.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bms.1200151009
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    Paper (German National Licenses)
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