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  • 1
    Electronic Resource
    Electronic Resource
    Regulation of the gating of the sheep cardiac sarcoplasmic reticulum ca2+-release channel by luminal Ca2+ (1994)
    Springer
    The journal of membrane biology 137 (1994), S. 215-226 
    Details
    ISSN: 1432-1424
    Keywords: Ca2+-release channels ; Sarcoplasmic reticulum ; Cardiac ; Sulmazole
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract We investigated the effects of changes in luminal [Ca2+] on the gating of native andpurified sheep cardiac sarcoplasmic reticulum (SR) Ca2+-release channels reconstituted intoplanar phospholipid bilayers. The open probability (P o )of channels activated solely by cytosolic Ca2+ was greater at positive than negative holding potentials. Channels activatedsolely by 10 μm cytosolic Ca2+ exhibited no change in steady-stateP o or in the relationship betweenP o and voltage when the luminal[Ca2+] was increased from nanomolar to millimolar concentrations. In the absence of activating concentrationsof cytosolic Ca2+, the channel can be activated by the phosphodiesterase inhibitor sulmazole (AR-L 115BS). However, cytosolicCa2+-independent activation of the channel by sulmazole requires luminal Ca2+. In the presence ofsulmazole, at picomolar luminal [Ca2+] the channel remains completely closed. Increasing the luminal [Ca2+]to millimolar levels markedly increases the P o via an increase in theduration of open events. The P o and duration of the sulmazole-activated, luminalCa2+-dependent channel openings are voltage dependent. In the presence of micromolar luminal Ca2+, theP o and duration of sulmazole-activated openings are greater atnegative voltages. However, at millimolar luminal [Ca2+], long openings are also observed at positive voltages and theP o appears to be similar at positive and negative voltages. Our findings indicate thatthe regulation of channel gating by luminal Ca2+ depends on the mechanism of channel activation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00232590
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  • 2
    Electronic Resource
    Electronic Resource
    Adenosine discriminates between the caffeine and adenine nucleotide sites on the sheep cardiac sarcoplasmic reticulum calcium-release channel (1994)
    Springer
    The journal of membrane biology 137 (1994), S. 169-177 
    Details
    ISSN: 1432-1424
    Keywords: Adenosine ; Sarcoplasmic reticulum ; Cardiac Ca2+-release channel ; Caffeine ; Adenine nucleotides
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Calcium-release channels of sheep cardiac sarcoplasmic reticulum were incorporated into phosphatidylethanolamine bilayers and single channel currents were recorded under voltage-clamp conditions. The effect of adenosine on single channel conductance and gating was investigated, as were the interactions between adenosine and caffeine and adenosine and α,β-methylene ATP. Addition of adenosine (0.5–5 mm) to the cytosolic but not the luminal side of the membrane increased the open probability of single calcium-activated calcium-release channels by increasing the frequency and duration of open events, yielding an EC50 of 0.75 mm at 10 μm activating Ca2+. Addition of 1 mm caffeine potentiated the effects of adenosine at 10 or 100 μm-activating cytosolic calcium, but had no effect on the inability of adenosine to activate the channel at 80 pmcalcium, suggesting discrete sites of action on the calcium-release channel for adenosine and caffeine. In contrast, addition of 100 μm α,β-methylene-ATP decreased single channel open probability in the presence of adenosine, suggesting that these compounds act on the same site on the channel. Activation of single channel opening by adenosine, or by adenosine together with caffeine, had no effect on single channel conductance or the Ca2+/Tris+ permeability ratio. Channels activated by adenosine were characteristically modified by ryanodine and blocked by μm ruthenium red or mm magnesium. These results show that adenosine activates the sheep cardiac sarcoplasmic reticulum Ca2+-release channel by increasing the frequency and duration of open events in a Ca2+-dependent manner. The receptor site on the channel for adenosine is distinct from that for caffeine but probably the same as that for adenine nucleotides.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00233486
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  • 3
    Electronic Resource
    Electronic Resource
    The gating of the sheep skeletal sarcoplasmic reticulum Ca2+-release channel is regulated by luminal Ca2+ (1995)
    Springer
    The journal of membrane biology 146 (1995), S. 133-144 
    Details
    ISSN: 1432-1424
    Keywords: Sarcoplasmic reticulum ; Skeletal muscle ; Calcium-release channel ; Ryanodine receptor ; Channel gating
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The effects of changes in luminal [Ca2+] have been investigated in sheep skeletal sarcoplasmic reticulum (SR) Ca2+-release channels after activation of the channels by different ligands from the cytosolic side of the channel. Native heavy SR membrane vesicles were incorporated into planar phospholipid bilayers under voltage-clamp conditions. Experiments were carried out in symmetrical 250 mm Cs+. Lifetime analysis indicates that channels activated solely by cytosolic Ca2+ exhibit at least two open and five closed states. The open events are very brief and are close to the minimum resolvable duration. When channels are activated solely by cytosolic Ca2+, luminal Ca2+ does not appear to exert any regulatory effect. The P 0 and duration of the open and closed lifetimes are unchanged. However, if channels are activated by ATP alone or by ATP plus cytosolic Ca2+, increases in luminal [Ca2+] produce marked increases in P 0 and in the duration of the open lifetimes. Our results demonstrate that maximum activation of the skeletal SR Ca2+-release channel by ATP cannot be obtained in the absence of millimolar luminal [Ca2+].
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00238004
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  • 4
    Electronic Resource
    Electronic Resource
    Thermal neutron captureγ-ray spectroscopy of59Ni and61Ni (1993)
    Springer
    The European physical journal 345 (1993), S. 143-153 
    Details
    ISSN: 1434-601X
    Keywords: 21.10.Ma ; 23.20.Lv ; 25.40.Lw ; 27.50.+ e
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Theγ-radiation emitted after thermal neutron capture in isotopically enriched58Ni and60Ni was measured at the ILL high flux reactor by means of Ge/NaI detectors operated in Compton suppression and pair spectrometer mode. The neutron binding energies were determined asB n (59Ni)=8999.15(23) keV and Bn(61Ni)=7820.07(20) keV; some 95% of the totalγ-ray fluxes through59,61Ni were assigned. Theγ-ray strength functions of the primary transitions and the level densities are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01293337
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  • 5
    Electronic Resource
    Electronic Resource
    Double neutron capture in62Ni (1992)
    Springer
    The European physical journal 343 (1992), S. 7-14 
    Details
    ISSN: 1434-601X
    Keywords: 21.10.Ma ; 23.20.Lv ; 25.40.Lw ; 27.50. + e
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract The γ-radiation following single and double neutron capture in isotopically enriched62Ni was studied at the high flux reactor of the Institut Laue-Langevin, using a pair and Compton suppressed germanium detector. Measurements before and after 170 d of breeding were performed. The γ-ray fluxes through63Ni and64Ni are discussed; several new levels and spin-parity assignments were found. On the basis of the known discrete levels and the low-energy neutron resonances, level density parameters were determined within the Constant Temperature Fermi Gas model. The neutron binding energies were measured asB n (63Ni)=6837.92(18) keV andB n (64Ni)=9657.64(24) keV. The63Ni (n, γ) cross section for reactor neutrons was measured to be σ=20 −2 +5 b.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01291590
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  • 6
    Electronic Resource
    Electronic Resource
    Gas chromatographic analysis of furaneol (1981)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 4 (1981), S. 421-422 
    Details
    ISSN: 0935-6304
    Keywords: Gas chromatography ; Capillary columns ; Unstable flavor compound ; Comparison of fused silica, glass, and metal columns ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240040815
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  • 7
    Electronic Resource
    Electronic Resource
    Preparation of robust tapered restrictors for capillary supercritical fluid chromatography (1988)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 11 (1988), S. 289-291 
    Details
    ISSN: 0935-6304
    Keywords: Supercritical fluid chromatography, SFC ; Capillary columns ; Restrictor ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240110314
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  • 8
    Electronic Resource
    Electronic Resource
    Supercritical fluid extraction / capillary supercritical fluid chromatography / Fourier transform infrared microspectrometry of polycyclic aromatic compounds in a coal tar pitch (1988)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 11 (1988), S. 766-775 
    Details
    ISSN: 0935-6304
    Keywords: Supercritical fluid extraction, SFE ; Supercritical fluid chromatography, SFC ; Fourier transform infrared microspectrometry, FTIR ; Narrow-bore capillary column ; Polycyclic aromatic compounds, PAC ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The combination of supercritical fluid extraction, high resolution capillary supercritical fluid chromatography, and Fourier transform infrared microspectrometry is described for the separation and identification of polycyclic aromatic hydrocarbons in a coal tar pitch. The variable solvating power of the supercritical fluid was utilized to selectively fractionate the sample. The fluid extract was decompressed through a frit restrictor into the sample cavity of a cooled microvalve injector, where the analytes were deposited and concentrated for subsequent chromatographic analysis. Several of the analytes separated in the chromatograph were collected on a potassium bromide disc at a solvent elimination inter-face for subsequent infrared analysis involving the use of an infra-red microscope accessory. The spectra obtained show the power of this detection technique for distinguishing between isomers.
    Additional Material: 11 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240111105
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  • 9
    Electronic Resource
    Electronic Resource
    The application of conversion electron Mössbauer spectroscopy (C.E.M.S.) to the study of corrosion inhibition in brass condenser tubes (1981)
    Weinheim [u.a.] : Wiley-Blackwell
    Materials and Corrosion/Werkstoffe und Korrosion 32 (1981), S. 302-305 
    Details
    ISSN: 0947-5117
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Description / Table of Contents: Anwendung der, „Conversion Electron Mössbauer Spectroscopy“ (C. E. M. S.) auf die Untersuchung der Inhibierung der Korrosion in Kondensatorrohren aus MessingBeschrieben wird der Aufbau eines einfachen Zählers zur Aufnahme des „Conversion Electron Mössbauer-Spektrums“ der inneren Oberfläche eines Rohres. Die Zählvorrichtung wird benutzt zur Untersuchung des Einflusses von Eisen-II-Sulfatzusatz als Inhibitor in Kühlwasser für Kondensatorrohre aus Messing. Aufgrund der Ergebnisse führt these Behandlung zur Bildung einer FeOOH-Schicht auf der inneren Rohroberfläche; die genaue Zusammensetzung der Schicht unterscheidet sich geringfügig je nach den örtlichen Verhältnissen.
    Notes: The construction is described of a simple counter for measuring the conversion electron Mössbauer spectrum of the inside surface of a tube. The counter is used to investigate the effects of adding ferrous sulphate to the cooling water of condensers with brass tubes to inhibit corrosion. It is concluded that the treatment leads to the formation on the inside of the tube of a layer of FeOOH, the exact nature of which depends slightly on the local conditions.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/maco.19810320704
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  • 10
    Electronic Resource
    Electronic Resource
    DNA Macroradicals produced by ultrasonic irradiation (1969)
    New York : Wiley-Blackwell
    Biopolymers 8 (1969), S. 555-558 
    Details
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bip.1969.360080414
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