ISSN:
1573-0972
Keywords:
Chitinase
;
marine bacteria
;
Vibrio sp.
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Abstract A chitinase was separated from the culture broth of Vibrio sp. 11211 isolated from sediment from the South China Sea. The chitinase was purified 18.3-fold with 33% recovery by ammonium sulphate precipitation and chromatography. The subunit molecular weight of the enzyme was estimated by SDS-PAGE to be about 30 kDa. The enzyme showed optimum pH at 6.5 and optimum temperature at 50 °C, and was stable in the pH range of 4 to 9 and at the temperature below 40 °C.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1008932217926
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