ISSN:
1437-160X
Keywords:
Glucocorticoid receptor
;
Chloramphenicol acetyltransferase
;
Transient transfection
;
Chinese hamster ovary cells
;
Transcription
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract To determine the relative potency of synthetic glucocorticoids, glucocorticoid receptor expressing cells were transfected with a hormone-inducible reporter gene, and were cultured in the presence of various glucocorticoid ligands. Hormonal inducbility was determined by means of a chloramphenicol acetyltransferase assay. Dexamethasone and prednisolone, as well as cortisol, induced the expression of the reporter gene in a dose-dependent fashion. The relative potency of each ligand was in this order when inducibility was quantitatively assessed. In conclusion, the transcription assay described here may be a convenient and alternative method to evaluate the relative potency of given glucocorticoids.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00302665
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