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  • Biolistic  (1)
  • Chloroplast genome  (1)
  • 1
    Digitale Medien
    Digitale Medien
    Stable transformation and regeneration of transgenic plants of Pinus radiata D. Don (1998)
    Springer
    Plant cell reports 17 (1998), S. 460-468 
    Details
    ISSN: 1432-203X
    Schlagwort(e): Key wordsPinus radiata ; Transformation ; Biolistic ; Embryogenic tissue
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract A biolistic particle delivery system was used to genetically transform embryogenic tissue of Pinus radiata. The introduced DNA contained a uidA reporter gene under the control of either the tandem CaMV 35S or the artificial Emu promoter, and the npt II selectable marker controlled by the CaMV 35S promoter. The average number of stable, geneticin-resistant lines recovered was 0.5 per 200 mg fresh weight bombarded tissue. Expression of the uidA reporter gene was detected histochemically and fluorimetrically in transformed embryogenic tissue and in derived mature somatic embryos and regenerated plants. The integration of uidA and npt II genes into the Pinus radiata genome was demonstrated using PCR amplification of the inserts and Southern hybridisation analysis. The expression of both genes in transformed tissue was confirmed by Northern hybridisation analysis. More than 150 transgenic Pinus radiata plants were produced from 20 independent transformation experiments with four different embryogenic clones.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s002990050426
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Restriction fragment length polymorphisms in the genusActinidia (Actinidiaceae) (1990)
    Springer
    Plant systematics and evolution 172 (1990), S. 193-203 
    Details
    ISSN: 1615-6110
    Schlagwort(e): Angiosperms ; Actinidiaceae ; Actinidia ; Chloroplast genome ; kiwifruit ; molecular evolution ; phylogenetic trees ; restriction fragment length polymorphism
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract A series of chloroplast and nuclear probes were used to examine restriction fragment length polymorphisms (RFLPs) in kiwifruit (Actinidia deliciosa) and three of its closest relatives. The four species fell into two pairs, withA. chinensis andA. deliciosa closely related but some distance away from the other two species,A. latifolia andA. eriantha. The results are consistent with the hypothesis that the diploid species,A. chinensis, is a precursor ofA. deliciosa, which is hexaploid.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00937806
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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    Artikel (Nationallizenzen)
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