ISSN:
1617-4623
Keywords:
Key wordsVf
;
Malus×domestica
;
Resistance gene
;
Chromosome walking
;
Inverse PCR
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract A positional cloning project was started in apple with the aim of isolating the Vf resistance gene of Malus floribunda 821. Vf confers resistance against apple scab, the most important disease in apple orchards. A chromosome walk starting from two molecular markers (M18-CAPS and AM19-SCAR) flanking Vf was performed, using a bacterial artificial chromosome (BAC) library containing inserts of the cultivar Florina, which is heterozygous for Vf. Thirteen BAC clones spanning the region between the two markers were identified in nine chromosome walking steps. The size of the resulting contig is approximately 550 kb. In order to map the Vf region in more detail, we analyzed over 2000 plants from different populations segregating for Vf with markers produced from BAC end sequences. In this way, we were able to restrict the possible location of the Vf gene to a minimum of five clones spanning an interval of approximately 350 kb.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s004380051154
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